Chinese medicine (2012). Volume: 7, Issue: 1. Pages: 8. Dirk W Lachenmeier, Yulia B Monakhova, Thomas Kuballa, Sigrid Lobell-Behrends, Sibylle Maixner, Matthias Kohl-Himmelseher, Asja Waldner, Christian Steffen et al.
ABSTRACT: BACKGROUND: Red yeast rice (i.e., rice fermented with Monascus spp.), as a food supplement, is claimed to be blood cholesterol-lowering. The red yeast rice constituent monacolin K, also known as lovastatin, is an inhibitor of the hydroxymethylglutaryl-CoA (HMG-CoA) reductase. This article aims to develop a sensitive nuclear magnetic resonance (NMR) method to determine the total statin content of red yeast rice products. METHODS: The total statin content was determined by a 400 MHz 1H NMR spectroscopic method, based on the integration of the multiplet at delta 5.37-5.32 ppm of a hydrogen at the hexahydronaphthalene moiety in comparison to an external calibration with lovastatin. The activity of HMG-CoA reductase was measured by a commercial spectrophotometric assay kit. RESULTS: The NMR detection limit for total statins was 6 mg/L (equivalent to 0.3 mg/capsule, if two capsules are dissolved in 50 mL ethanol). The relative standard deviations were consistently lower than 11%. The total statin concentrations of five red yeast rice supplements were between 1.5 and 25.2 mg per specified daily dose. A dose-dependent inhibition of the HMG-CoA reductase enzyme activity by the red yeast rice products was demonstrated. CONCLUSION: A simple and direct NMR assay was developed to determine the total statin content in red yeast rice. The assay can be applied for the determination of statin content for the regulatory control of red yeast rice products.
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[NMR paper] NMR evaluation of total statin content and HMG-CoA reductase inhibition in red yeast rice (Monascus spp.) food supplements.
From Mendeley Biomolecular NMR group:
NMR evaluation of total statin content and HMG-CoA reductase inhibition in red yeast rice (Monascus spp.) food supplements.
Chinese medicine (2012). Volume: 7, Issue: 1. Pages: 8. Dirk W Lachenmeier, Yulia B Monakhova, Thomas Kuballa, Sigrid Lobell-Behrends, Sibylle Maixner, Matthias Kohl-Himmelseher, Asja Waldner, Christian Steffen et al.
ABSTRACT: BACKGROUND: Red yeast rice (i.e., rice fermented with Monascus spp.), as a food supplement, is claimed to be blood cholesterol-lowering. The red yeast rice constituent monacolin K, also known as...
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10-19-2012 10:22 AM
[NMR paper] NMR evaluation of total statin content and HMG-CoA reductase inhibition in red yeast rice (Monascus spp.) food supplements.
From Mendeley Biomolecular NMR group:
NMR evaluation of total statin content and HMG-CoA reductase inhibition in red yeast rice (Monascus spp.) food supplements.
Chinese medicine (2012). Volume: 7, Issue: 1. Pages: 8. Dirk W Lachenmeier, Yulia B Monakhova, Thomas Kuballa, Sigrid Lobell-Behrends, Sibylle Maixner, Matthias Kohl-Himmelseher, Asja Waldner, Christian Steffen et al.
ABSTRACT: BACKGROUND: Red yeast rice (i.e., rice fermented with Monascus spp.), as a food supplement, is claimed to be blood cholesterol-lowering. The red yeast rice constituent monacolin K, also known as...
nmrlearner
Journal club
0
10-12-2012 09:58 AM
[NMR paper] NMR evaluation of total statin content and HMG-CoA reductase inhibition in red yeast rice (Monascus spp.) food supplements.
From Mendeley Biomolecular NMR group:
NMR evaluation of total statin content and HMG-CoA reductase inhibition in red yeast rice (Monascus spp.) food supplements.
Chinese medicine (2012). Volume: 7, Issue: 1. Pages: 8. Dirk W Lachenmeier, Yulia B Monakhova, Thomas Kuballa, Sigrid Lobell-Behrends, Sibylle Maixner, Matthias Kohl-Himmelseher, Asja Waldner, Christian Steffen et al.
ABSTRACT: BACKGROUND: Red yeast rice (i.e., rice fermented with Monascus spp.), as a food supplement, is claimed to be blood cholesterol-lowering. The red yeast rice constituent monacolin K, also known as...
nmrlearner
Journal club
0
08-24-2012 08:01 PM
NMR assignments of ubiquitin fold domain (UFD) in SUMO-activating enzyme subunit 2 from rice.
NMR assignments of ubiquitin fold domain (UFD) in SUMO-activating enzyme subunit 2 from rice.
NMR assignments of ubiquitin fold domain (UFD) in SUMO-activating enzyme subunit 2 from rice.
Biomol NMR Assign. 2011 Apr 27;
Authors: Suzuki R, Tsuchiya W, Shindo H, Yamazaki T
The small ubiquitin-related modifier (SUMO) is a ubiquitin-like post-translational modifier that alters the localization, activity, or stability of many proteins. In the sumoylation process, an activated SUMO is transferred from SUMO-activating enzyme E1 complex (SAE1/SAE2) to...
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04-28-2011 03:12 PM
[NMR paper] 1H(C) and 1H(N) total NOE correlations in a single 3D NMR experiment. 15N and 13C tim
1H(C) and 1H(N) total NOE correlations in a single 3D NMR experiment. 15N and 13C time-sharing in t1 and t2 dimensions for simultaneous data acquisition.
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J Biomol NMR. 2003 Nov;27(3):193-203
Authors: Xia Y, Yee A, Arrowsmith CH, Gao X
Simultaneous data acquisition in time-sharing (TS) multi-dimensional NMR experiments has been shown an effective means to reduce experimental...
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[NMR paper] High precision NMR structure and function of the RING-H2 finger domain of EL5, a rice
High precision NMR structure and function of the RING-H2 finger domain of EL5, a rice protein whose expression is increased upon exposure to pathogen-derived oligosaccharides.
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J Biol Chem. 2003 Apr 25;278(17):15341-8
Authors: Katoh S, Hong C, Tsunoda Y, Murata K, Takai R, Minami E, Yamazaki T, Katoh E
EL5, a RING-H2 finger protein, is rapidly induced by...
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[NMR paper] Determination of the casein content in bovine milk by 31P-NMR.
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Authors: Belloque J, Ramos M
The relative proportion of caseins to total protein is a parameter that can be used to control the protein quality in standardised milk, an increasing tendency in dairy industries. 31P-NMR was used to analyse the casein content of milk, by the quantitation of the area under the resonances belonging to SerP, and using methylenediphosphonic...
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[NMR paper] Quantitative evaluation of water content in a solid protein by deuterium NMR.
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Biochim Biophys Acta. 1992 Feb 26;1119(2):178-84
Authors: Tamura A, Akasaka K
A method for evaluating absolute water content in a solid protein based on deuterium NMR measurements in solution is described. By dissolving the hydrated solid protein, which has been specifically deuterium-labeled, into deuterium-depleted water and by comparing the deuterium NMR signal intensity...
NMR evaluation of total statin content and HMG-CoA reductase inhibition in red yeast rice (Monascus spp.) food supplements.
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