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Default NMR-Detected Brownian Dynamics of ?B-Crystallin over a Wide Range of Concentrations.

NMR-Detected Brownian Dynamics of ?B-Crystallin over a Wide Range of Concentrations.

NMR-Detected Brownian Dynamics of ?B-Crystallin over a Wide Range of Concentrations.

Biophys J. 2015 Jan 6;108(1):98-106

Authors: Roos M, Link S, Balbach J, Krushelnitsky A, Saalwächter K

Abstract
Knowledge about the global translational and rotational motion of proteins under crowded conditions is highly relevant for understanding the function of proteins in*vivo. This holds in particular for human ?B-crystallin, which is strongly crowded in*vivo and inter alia responsible for preventing cataracts. Quantitative information on translational and rotational diffusion is not readily available, and we here demonstrate an approach that combines pulsed-field-gradient NMR for translational diffusion and proton T1?/T2 relaxation-time measurements for rotational diffusion, thus overcoming obstacles encountered in previous studies. The relaxation times measured at variable temperature provide a quantitative measure of the correlation function of protein tumbling, which cannot be approximated by a single exponential, because two components are needed for a minimal and adequate description of the data. We find that at high protein concentrations, rotational diffusion is decoupled from translational diffusion, the latter following the macroscopic viscosity change almost quantitatively, resembling the behavior of spherical colloids. Analysis of data reported in the literature shows that well-packed globular proteins follow a scaling relation between the hydrodynamic radius and the molar mass, Rh ~ M(1/d), with a fractal dimension of d ~ 2.5 rather than 3. Despite its oligomeric nature, Rh of ?B-crystallin as derived from both NMR methods is found to be fully consistent with this relation.


PMID: 25564856 [PubMed - as supplied by publisher]



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