NMR Characterization of a 72 kDa transcription factor using differential isotopic labeling.
 

NMR Characterization of a 72 kDa transcription factor using differential isotopic labeling.

Related Articles NMR Characterization of a 72 kDa transcription factor using differential isotopic labeling.

Protein Sci. 2015 Dec 8;

Authors: Mukherjee SP, Borin B, Quintas PO, Dyson HJ

Abstract
NF-?B is a major transcription factor that mediates a number of cellular signaling pathways. Crystal structure analysis gives an incomplete picture of the behavior of the protein, particularly in the free state; free monomers or dimers of NF-?B have never been crystallized. NMR analysis gives insights into the structure and dynamics of the protein in solution, but a necessary first step is the assignment of resonances. The size of the heterodimer of the Rel homology regions of the NF-?B monomers p65 and p50 (72 kDa) prohibits the straightforward use of triple-resonance spectroscopy to obtain the assignments. However, the dynamic nature of the free heterodimer, in particular the independence of the DNA-binding and dimerization domains of each monomer, allow the assignments made on differentially-labeled smaller domains to be mapped successfully onto the spectrum of the larger full-length RHR. Problematic areas such as the p65 nuclear localization sequence, which is disordered in the free protein, can be approached by residue-specific labeling and comparison with previously-published spectra of a short peptide with the same sequence. Overall, this NMR analysis of NF-?B has given valuable insights into the highly dynamic nature of the free state, which is likely to play an important role in the functional cycle of NF-?B in the cell. This article is protected by copyright. All rights reserved.


PMID: 26647230 [PubMed - as supplied by publisher]



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