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Default NMR-based structural characterization of large protein-ligand interactions.

NMR-based structural characterization of large protein-ligand interactions.

Related Articles NMR-based structural characterization of large protein-ligand interactions.

J Biomol NMR. 2002 Feb;22(2):165-73

Authors: Pellecchia M, Meininger D, Dong Q, Chang E, Jack R, Sem DS

Genomic research on target identification and validation has created a great need for methods that rapidly provide detailed structural information on protein-ligand interactions. We developed a suite of NMR experiments as rapid and efficient tools to provide descriptive structural information on protein-ligand complexes. The methods work with large proteins and in particular cases also without the need for a complete three-dimensional structure. We will show applications with two tetrameric enzymes of 120 and 170 kDa.

PMID: 11883777 [PubMed - indexed for MEDLINE]



Source: PubMed
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