NMR-based structural biology enhanced by dynamic nuclear polarization at high magnetic field.
 

NMR-based structural biology enhanced by dynamic nuclear polarization at high magnetic field.

http://www.bionmr.com//www.ncbi.nlm....ringerlink.gif Related Articles NMR-based structural biology enhanced by dynamic nuclear polarization at high magnetic field.

J Biomol NMR. 2014 Nov;60(2-3):157-68

Authors: Koers EJ, van der Cruijsen EA, Rosay M, Weingarth M, Prokofyev A, Sauvée C, Ouari O, van der Zwan J, Pongs O, Tordo P, Maas WE, Baldus M

Abstract
Dynamic nuclear polarization (DNP) has become a powerful method to enhance spectroscopic sensitivity in the context of magnetic resonance imaging and nuclear magnetic resonance spectroscopy. We show that, compared to DNP at lower field (400*MHz/263*GHz), high field DNP (800*MHz/527*GHz) can significantly enhance spectral resolution and allows exploitation of the paramagnetic relaxation properties of DNP polarizing agents as direct structural probes under magic angle spinning conditions. Applied to a membrane-embedded K(+) channel, this approach allowed us to refine the membrane-embedded channel structure and revealed conformational substates that are present during two different stages of the channel gating cycle. High-field DNP thus offers atomic insight into the role of molecular plasticity during the course of biomolecular function in a complex cellular environment.


PMID: 25284462 [PubMed - indexed for MEDLINE]



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