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Default NMR-based structural biology enhanced by dynamic nuclear polarization at high magnetic field

NMR-based structural biology enhanced by dynamic nuclear polarization at high magnetic field

Abstract

Dynamic nuclear polarization (DNP) has become a powerful method to enhance spectroscopic sensitivity in the context of magnetic resonance imaging and nuclear magnetic resonance spectroscopy. We show that, compared to DNP at lower field (400*MHz/263*GHz), high field DNP (800*MHz/527*GHz) can significantly enhance spectral resolution and allows exploitation of the paramagnetic relaxation properties of DNP polarizing agents as direct structural probes under magic angle spinning conditions. Applied to a membrane-embedded K+ channel, this approach allowed us to refine the membrane-embedded channel structure and revealed conformational substates that are present during two different stages of the channel gating cycle. High-field DNP thus offers atomic insight into the role of molecular plasticity during the course of biomolecular function in a complex cellular environment.



Source: Journal of Biomolecular NMR
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