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-   -   [NMR paper] NMR analysis of carbohydrate-binding interactions in solution: an approach using analysis of saturation transfer difference NMR spectroscopy. (http://www.bionmr.com/forum/journal-club-9/nmr-analysis-carbohydrate-binding-interactions-solution-approach-using-analysis-saturation-transfer-difference-nmr-spectroscopy-22049/)

nmrlearner 04-09-2015 03:47 AM

NMR analysis of carbohydrate-binding interactions in solution: an approach using analysis of saturation transfer difference NMR spectroscopy.
 
NMR analysis of carbohydrate-binding interactions in solution: an approach using analysis of saturation transfer difference NMR spectroscopy.

http://www.bionmr.com//www.ncbi.nlm....ringerlink.gif Related Articles NMR analysis of carbohydrate-binding interactions in solution: an approach using analysis of saturation transfer difference NMR spectroscopy.

Methods Mol Biol. 2014;1200:501-9

Authors: Hemmi H

Abstract
One of the most commonly used ligand-based NMR methods for detecting ligand binding is saturation transfer difference (STD) nuclear magnetic resonance (NMR) spectroscopy. The STD NMR method is an invaluable technique for assessing carbohydrate-lectin interactions in solution, because STD NMR can be used to detect weak ligand binding (Kd ca. 10(-3)-10(-8) M). STD NMR spectra identify the binding epitope of a carbohydrate ligand when bound to lectin. Further, the STD NMR method uses 1H-detected NMR spectra of only the carbohydrate, and so only small quantities of non-labeled lectin are required. In this chapter, I describe a protocol for the STD NMR method, including the experimental procedures used to acquire, process, and analyze STD NMR data, using STD NMR studies for methyl-?-D-galactopyranoside (?-Me-Gal) binding to the C-terminal domain of an R-type lectin from earthworm (EW29Ch) as an example.


PMID: 25117260 [PubMed - indexed for MEDLINE]



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