NMR paper New approaches to track chemoenzymatic loading reactions of NRPS carrier protein domains using NMR

		BioNMR > Educational resources > Journal club
[NMR paper] New approaches to track chemoenzymatic loading reactions of NRPS carrier protein domains using NMR

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

View First Unread

Thread Tools

Search this Thread

Rate Thread

Display Modes

02-15-2023, 11:58 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,174

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

New approaches to track chemoenzymatic loading reactions of NRPS carrier protein domains using NMR

New approaches to track chemoenzymatic loading reactions of NRPS carrier protein domains using NMR

No abstract

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[ASAP] Structural Basis of Transient Interactions of Acyltransferase VinK with the Loading Acyl Carrier Protein of the Vicenistatin Modular Polyketide Synthase Structural Basis of Transient Interactions of Acyltransferase VinK with the Loading Acyl Carrier Protein of the Vicenistatin Modular Polyketide Synthase https://pubs.acs.org/cms/10.1021/acs.biochem.2c00645/asset/images/medium/bi2c00645_0005.gif Biochemistry DOI: 10.1021/acs.biochem.2c00645 More...	nmrlearner	Journal club	0	12-19-2022 01:33 AM
[NMR paper] FRET Monitoring of a Nonribosomal Peptide Synthetase Elongation Module Reveals Carrier Protein Shuttling Between Catalytic Domains FRET Monitoring of a Nonribosomal Peptide Synthetase Elongation Module Reveals Carrier Protein Shuttling Between Catalytic Domains Angewandte Chemie International Edition, Accepted Article. More...	nmrlearner	Journal club	0	09-30-2022 04:26 AM
[NMR paper] NMR as a readout to monitor and restore the integrity of complex chemoenzymatic reactions NMR as a readout to monitor and restore the integrity of complex chemoenzymatic reactions The non-invasive nature of NMR offers a means to monitor biochemical reactions in situ at the atomic level. We harness this advantage to monitor a complex chemoenzymatic reaction that sequentially modifies reagents and loads the product on a nonribosomal peptide synthetase carrier protein. We present a protocol including a pulse sequence that permits to assess both the integrity of reagents and the completion of each step in the reaction, thus alleviating otherwise time-consuming and costly... ...	nmrlearner	Journal club	0	07-19-2022 05:10 PM
(1)H,(13)C and (15)N chemical shift assignments of the SUD domains of SARS-CoV-2 non-structural protein 3c: "The SUD-M and SUD-C domains" - DocWire News (1)H,(13)C and (15)N chemical shift assignments of the SUD domains of SARS-CoV-2 non-structural protein 3c: "The SUD-M and SUD-C domains" - DocWire News (1)H,(13)C and (15)N chemical shift assignments of the SUD domains of SARS-CoV-2 non-structural protein 3c: "The SUD-M and SUD-C domains" DocWire News Read here	nmrlearner	Online News	0	01-13-2021 08:38 AM
[NMR paper] Adaptive Chemoenzymatic Microreactors Composed of Inorganic Nanoparticles and Bioinspired Intrinsically Disordered Proteins Adaptive Chemoenzymatic Microreactors Composed of Inorganic Nanoparticles and Bioinspired Intrinsically Disordered Proteins Angewandte Chemie International Edition, Volume 59, Issue 21, Page 8138-8142, May 18, 2020. More...	nmrlearner	Journal club	0	05-12-2020 08:32 PM
Acyl Carrier Protein Cyanylation Delivers a KetoacylSynthase–Carrier Protein Cross-Link Acyl Carrier Protein Cyanylation Delivers a KetoacylSynthase–Carrier Protein Cross-Link http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.7b00219/20170508/images/medium/bi-2017-00219v_0003.gif Biochemistry DOI: 10.1021/acs.biochem.7b00219 http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/bichaw/~4/At3bkgocnak More...	nmrlearner	Journal club	0	05-09-2017 12:37 AM
Variability and conservation of structural domains in divide-and-conquer approaches Variability and conservation of structural domains in divide-and-conquer approaches Abstract The use of protein building blocks for the structure determination of multidomain proteins and proteinâ??protein complexes, also known as the â??divide and conquerâ?? approach, is an important strategy for obtaining protein structures. Atomic-resolution X-ray or NMR data of the individual domains are combined with lower-resolution electron microscopy maps or X-ray data of the full-length protein or the protein complex. Doing so, it is often assumed that the...	nmrlearner	Journal club	0	05-30-2016 01:15 PM
[NMR paper] An NMR method to probe molecular influences of substrate loading in NRPS carrier proteins. An NMR method to probe molecular influences of substrate loading in NRPS carrier proteins. http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles An NMR method to probe molecular influences of substrate loading in NRPS carrier proteins. Biochemistry. 2015 Jan 26; Authors: Goodrich AC, Frueh DP Abstract Carrier proteins (CPs) play a central role in nonribosomal peptide (NRP) synthesis by shuttling covalently attached substrates between active sites....	nmrlearner	Journal club	0	01-28-2015 03:22 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off