Native mass spectrometry of photosynthetic pigment-protein complexes.
 

Native mass spectrometry of photosynthetic pigment-protein complexes.

http://www.ncbi.nlm.nih.gov/corehtml...PubMedLink.gif Related Articles Native mass spectrometry of photosynthetic pigment-protein complexes.

FEBS Lett. 2013 Jan 18;

Authors: Zhang H, Cui W, Gross ML, Blankenship RE

Abstract
Native mass spectrometry (MS), or as is sometimes called "native electrospray ionization" allows proteins in their native or near-native states in solution to be introduced into the gas phase and interrogated by mass spectrometry. This approach is now a powerful tool to investigate protein complexes. This article reviews the background of native MS of protein complexes and describes its strengths, taking photosynthetic pigment-protein complexes as examples. Native MS can be utilized in combination with other MS-based approaches to obtain complementary information to that provided by tools such as X-ray crystallography and NMR spectroscopy to understand the structure-function relationships of protein complexes. When additional information beyond that provided by native MS is required, other MS-based strategies can be successfully applied to augment the results of native MS.


PMID: 23337874 [PubMed - as supplied by publisher]



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