Related ArticlesPreparation of Caveolin-1 for NMR Spectroscopy Experiments.
Methods Mol Biol. 2020;2169:137-147
Authors: Plucinsky SM, Julien JA, Glover KJ
Abstract
Caveolin-1 is a 20.5*kDa integral membrane protein that is involved in a myriad of cellular processes including signal transduction, relieving mechano-stresses on the cell, endocytosis, and most importantly caveolae formation. As a consequence, there is intense interest in characterizing caveolin-1 structurally. Out of the many available structural techniques, nuclear magnetic resonance (NMR) spectroscopy is particularly well suited to investigations on integral membrane proteins like caveolin-1 that have significant unstructured regions and unusual topologies. However, the technique requires relatively large amounts of protein (i.e. concentrations in the 0.5-5*mM range), and obtaining these amounts can be difficult especially for highly hydrophobic membrane proteins such as caveolin-1. Herein, we describe a robust protocol for the preparation of caveolin-1 for structural studies using NMR.
DNP NMR spectroscopy of cross-linked organic polymers: rational guidelines towards optimal sample preparation #DNPNMR
From The DNP-NMR Blog:
DNP NMR spectroscopy of cross-linked organic polymers: rational guidelines towards optimal sample preparation #DNPNMR
Tanaka, Shinji, Wei-Chih Liao, Atsuko Ogawa, Kazuhiko Sato, and Christophe Copéret. “DNP NMR Spectroscopy of Cross-Linked Organic Polymers: Rational Guidelines towards Optimal Sample Preparation.” Physical Chemistry Chemical Physics 22, no. 6 (2020): 3184–90.
https://doi.org/10.1039/C9CP05208A.
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[NMR paper] Sample Preparation and Technical Setup for NMR Spectroscopy with Integral Membrane Proteins.
Sample Preparation and Technical Setup for NMR Spectroscopy with Integral Membrane Proteins.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles Sample Preparation and Technical Setup for NMR Spectroscopy with Integral Membrane Proteins.
Methods Mol Biol. 2020;2127:373-396
Authors: Kaur H, Grahl A, Hartmann JB, Hiller S
Abstract
NMR spectroscopy is a method of choice to characterize structure, function, and dynamics of integral...
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03-03-2020 11:18 PM
[NMR paper] Filamentous Bacteriophage Viruses: Preparation, Magic-Angle Spinning Solid-State NMR Experiments, and Structure Determination.
Filamentous Bacteriophage Viruses: Preparation, Magic-Angle Spinning Solid-State NMR Experiments, and Structure Determination.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles Filamentous Bacteriophage Viruses: Preparation, Magic-Angle Spinning Solid-State NMR Experiments, and Structure Determination.
Methods Mol Biol. 2018;1688:67-97
Authors: Morag O, Sgourakis NG, Abramov G, Goldbourt A
Abstract
Filamentous bacteriophages are...
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[NMR paper] Preparation of Amyloid Fibrils for Magic-Angle Spinning Solid-State NMR Spectroscopy.
Preparation of Amyloid Fibrils for Magic-Angle Spinning Solid-State NMR Spectroscopy.
Related Articles Preparation of Amyloid Fibrils for Magic-Angle Spinning Solid-State NMR Spectroscopy.
Methods Mol Biol. 2016;1345:173-83
Authors: Tuttle MD, Courtney JM, Barclay AM, Rienstra CM
Abstract
Solid-state NMR spectroscopy (SSNMR) is an established and invaluable tool for the study of amyloid fibril structure with atomic-level detail. Optimization of the homogeneity and concentration of fibrils enhances the resolution and sensitivity...
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[NMR paper] Structural study of caveolin-1 intramembrane domain by CD and NMR.
Structural study of caveolin-1 intramembrane domain by CD and NMR.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-2250-98-WileyOnlineLibrary-Button_120x27px_FullText.gif Related Articles Structural study of caveolin-1 intramembrane domain by CD and NMR.
Biopolymers. 2014 Dec 4;
Authors: Yang G, Dong Z, Xu H, Wang C, Li H, Li Z, Li F
Abstract
Caveolin-1 is a main structural component of caveolae and essential for the invagination of caveolae by forming a hairpin structure in the...
Preparation of a functional GABARAP-lipid conjugate in nanodiscs and its investigation by solution NMR spectroscopy.
Preparation of a functional GABARAP-lipid conjugate in nanodiscs and its investigation by solution NMR spectroscopy.
Preparation of a functional GABARAP-lipid conjugate in nanodiscs and its investigation by solution NMR spectroscopy.
Chembiochem. 2010 Sep 24;11(14):1967-70
Authors: Ma P, Mohrlüder J, Schwarten M, Stoldt M, Singh SK, Hartmann R, Pacheco V, Willbold D
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[NMR paper] Preparation, characterization, and NMR spectroscopy of encapsulated proteins dissolve
Preparation, characterization, and NMR spectroscopy of encapsulated proteins dissolved in low viscosity fluids.
Related Articles Preparation, characterization, and NMR spectroscopy of encapsulated proteins dissolved in low viscosity fluids.
J Biomol NMR. 2003 Apr;25(4):313-23
Authors: Babu CR, Flynn PF, Wand AJ
Encapsulating a protein in a reverse micelle and dissolving it in a low-viscosity solvent can lower the rotational correlation time of a protein and thereby provides a novel strategy for studying proteins in a variety of contexts. The...
Preparation of Caveolin-1 for NMR Spectroscopy Experiments.
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