Monitoring the Disassembly of Virus-like Particles by 19F-NMR.
 

Monitoring the Disassembly of Virus-like Particles by 19F-NMR.

Related Articles Monitoring the Disassembly of Virus-like Particles by 19F-NMR.

J Am Chem Soc. 2017 Mar 28;:

Authors: Leung RL, Robinson MD, Ajabali AA, Karunanithy G, Lyons B, Raj R, Raoufmoghaddam S, Mohammed S, Claridge TD, Baldwin AJ, Davis BG

Abstract
Virus-like particles (VLPs) are stable protein cages derived from virus coats. They have been used extensively as biomolecular platforms, e.g. nanocarriers or vaccines, but a convenient in situ technique is lacking for tracking functional status. Here, we present a simple way to monitor disassembly of 19F-labeled VLPs derived from bacteriophage Q? by 19F NMR. Analysis of resonances, under a range of conditions, allowed determination not only of the particle as fully assembled but also as disas-sembled, as well as detection of a degraded state upon digestion by cells. This in turn allowed mutational redesign of disassembly and testing in both bacterial and mammalian systems as a strategy for the creation of putative, targeted-VLP delivery systems.


PMID: 28350443 [PubMed - as supplied by publisher]



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