Monitoring ATP hydrolysis and ATPase inhibitor screening using (1)H NMR.
 

Monitoring ATP hydrolysis and ATPase inhibitor screening using (1)H NMR.

Related Articles Monitoring ATP hydrolysis and ATPase inhibitor screening using (1)H NMR.

Chem Commun (Camb). 2014 Aug 29;

Authors: Guo B, Gurel PS, Shu R, Higgs HN, Pellegrini M, Mierke DF

Abstract
We present a versatile method to characterize ATPase and kinase activities and discover new inhibitors of these proteins. The proton NMR-based assay directly monitors ATP turnover and is easy to implement, requires no additional reagents and can potentially be applied to GTP. We validated the method's accuracy, applied it to the monitoring of ATP turnover by actin and to the screening of ATPase inhibitors, and showed that it is also applicable for the monitoring of GTP hydrolysis.


PMID: 25170530 [PubMed - as supplied by publisher]



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