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Default Modification of Encapsulation Pressure of Reverse Micelles in Liquid Ethane

Modification of Encapsulation Pressure of Reverse Micelles in Liquid Ethane


Publication year: 2011
Source: Journal of Magnetic Resonance, In Press, Accepted Manuscript, Available online 20 June 2011

Ronald W., Peterson , Nathaniel V., Nucci , A., Joshua Wand

A central motivation for employing samples of encapsulated proteins dissolved in low viscosity fluids for high resolution NMR spectroscopy is to benefit from the superior performance afforded by the faster macromolecular rotation of the encapsulated protein than it has in free aqueous solution. Encapsulation of proteins within the protective aqueous core of a reverse micelle particle allows the use of ultra-low viscosity liquid ethane as solvent. Preparation of such samples requires significant pressure and the pressure-sensitivity of the viscosity of liquid ethane therefore becomes problematic. Here we survey a variety of additives for their ability to reduce the pressure necessary...

Graphical abstract

*Graphical abstract:**Highlights:*? Dissolution of encapsulated proteins in low viscosity fluids reduces the tumbling time ? The tumbling of the reverse micelle particle is governed by solvent viscosity ? Liquid ethane is a promising solvent but its viscosity is pressure dependent ? Additive molecules that reduce the encapsulation pressure are identified




Source: Journal of Magnetic Resonance
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