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Default A methyl-TROSY based 13C relaxation dispersion NMR experiment for studies of chemical exchange in proteins

A methyl-TROSY based 13C relaxation dispersion NMR experiment for studies of chemical exchange in proteins

A methyl Transverse Relaxation Optimized Spectroscopy (methyl-TROSY) based, multiple quantum (MQ) ^(13)C Carr-Purcell-Meiboom-Gill (CPMG) relaxation dispersion NMR experiment is described. The experiment is derived from the previously developed MQ ^(13)C-¹H CPMG scheme (Korzhnev in J Am Chem Soc 126: 3964-73, 2004) supplemented with a CPMG train of refocusing ¹H pulses applied with constant frequency and synchronized with the ^(13)C CPMG pulse train. The optimal ¹H 'decoupling' scheme that...

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