Methods for Structure Determination of SH2 Domain-Phosphopeptide Complexes by NMR
 

Methods for Structure Determination of SH2 Domain-Phosphopeptide Complexes by NMR

Nuclear magnetic resonance (NMR) spectroscopy is a powerful technique to solve the structure of biomolecular complexes at atomic resolution in solution. Small proteins such as Src-homology 2 (SH2) domains have fast tumbling rates and long-lived NMR signals, making them particularly suited to be studied by standard NMR methods. SH2 domains are modular proteins whose function is the recognition of sequences containing phosphotyrosines. In this chapter, we describe the application of NMR to assess...

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