Conventional approaches to study ligand-receptor interactions using solution-state NMR often involve laborious sample preparation, isotopic labeling, and receptor reconstitution. Each of these steps remains challenging for membrane proteins such as G protein-coupled receptors (GPCRs). Here we introduce a combinational approach integrating NMR and homogenized membrane nano-discs preparation to characterize the ligand-GPCR interactions. The approach will have a great potential for drug screening...
[NMR paper] Biphasic activation of beta-arrestin 1 upon interaction with a GPCR revealed by methyl-TROSY NMR
Biphasic activation of beta-arrestin 1 upon interaction with a GPCR revealed by methyl-TROSY NMR
?-arrestins (?arrs) play multifaceted roles in the function of G protein-coupled receptors (GPCRs). ?arrs typically interact with phosphorylated C-terminal tail (C tail) and transmembrane core (TM core) of GPCRs. However, the effects of the C tail- and TM core-mediated interactions on the conformational activation of ?arrs have remained elusive. Here, we show the conformational changes for ?arr activation upon the C tail- and TM core-mediated interactions with a prototypical GPCR by nuclear......
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[NMR paper] Probing transient non-native states in amyloid beta fiber elongation by NMR.
Probing transient non-native states in amyloid beta fiber elongation by NMR.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.rsc.org-images-entities-char_z_RSClogo.gif Related Articles Probing transient non-native states in amyloid beta fiber elongation by NMR.
Chem Commun (Camb). 2019 Apr 11;55(31):4483-4486
Authors: Brender JR, Ghosh A, Kotler SA, Krishnamoorthy J, Bera S, Morris V, Sil TB, Garai K, Reif B, Bhunia A, Ramamoorthy A
Abstract
Using NMR to probe transient binding of A?1-40 monomers to...
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04-19-2019 10:31 AM
Selective High-Resolution Detection of Membrane Protein-Ligand Interaction in Native Membranes Using Trityl-Nitroxide PELDOR
From The DNP-NMR Blog:
Selective High-Resolution Detection of Membrane Protein-Ligand Interaction in Native Membranes Using Trityl-Nitroxide PELDOR
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Joseph, B., et al., Selective High-Resolution Detection of Membrane Protein-Ligand Interaction in Native Membranes Using Trityl-Nitroxide PELDOR. Angew Chem Int Ed Engl, 2016. 55(38): p. 11538-42.
https://www.ncbi.nlm.nih.gov/pubmed/27511025
Probing ground and excited states of phospholamban in model and native lipid membranes by magic angle spinning NMR spectroscopy.
Probing ground and excited states of phospholamban in model and native lipid membranes by magic angle spinning NMR spectroscopy.
Probing ground and excited states of phospholamban in model and native lipid membranes by magic angle spinning NMR spectroscopy.
Biochim Biophys Acta. 2011 Aug 3;
Authors: Gustavsson M, Traaseth NJ, Veglia G
In this paper, we analyzed the ground and excited states of phospholamban (PLN), a membrane protein that regulates sarcoplasmic reticulum calcium ATPase (SERCA), in different membrane mimetic environments....
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08-16-2011 01:19 PM
[NMR paper] Probing membrane surfaces and the location of membrane-embedded peptides by (13)C MAS
Probing membrane surfaces and the location of membrane-embedded peptides by (13)C MAS NMR using lanthanide ions.
Related Articles Probing membrane surfaces and the location of membrane-embedded peptides by (13)C MAS NMR using lanthanide ions.
J Magn Reson. 1999 Dec;141(2):335-9
Authors: Gröbner G, Glaubitz C, Watts A
A simple but efficient (13)C MAS NMR method is presented for the determination of the location of embedded molecules such as peptides relative to biological membrane surfaces by exploiting the interaction with paramagnetic...
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11-18-2010 08:31 PM
[NMR paper] The native state of apomyoglobin described by proton NMR spectroscopy: interaction wi
The native state of apomyoglobin described by proton NMR spectroscopy: interaction with the paramagnetic probe HyTEMPO and the fluorescent dye ANS.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles The native state of apomyoglobin described by proton NMR spectroscopy: interaction with the paramagnetic probe HyTEMPO and the fluorescent dye ANS.
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08-22-2010 03:33 AM
[NMR paper] The native state of apomyoglobin described by proton NMR spectroscopy: interaction wi
The native state of apomyoglobin described by proton NMR spectroscopy: interaction with the paramagnetic probe HyTEMPO and the fluorescent dye ANS.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles The native state of apomyoglobin described by proton NMR spectroscopy: interaction with the paramagnetic probe HyTEMPO and the fluorescent dye ANS.
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An in-membrane NMR spectroscopic approach probing native ligand-GPCR interaction
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