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-   -   [NMR paper] The measurement of binding affinities by NMR chemical shift perturbation (http://www.bionmr.com/forum/journal-club-9/measurement-binding-affinities-nmr-chemical-shift-perturbation-29312/)

nmrlearner 08-04-2022 01:03 AM

The measurement of binding affinities by NMR chemical shift perturbation
 
The measurement of binding affinities by NMR chemical shift perturbation

We have carried out chemical shift perturbation titrations on three contrasting proteins. The resulting chemical shifts have been analysed to determine the best way to fit the data, and it is concluded that a simultaneous fitting of all raw shift data to a single dissociation constant is both the most accurate and the most precise method. It is shown that the optimal weighting of ^(15)N chemical shifts to ¹H chemical shifts is protein dependent, but is around the consensus value of 0.14. We show...

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