Publication year: 2010 Source: Journal of Magnetic Resonance, In Press, Accepted Manuscript, Available online 24 September 2010
Fabien, Ferrage , Amy, Reichel , Shibani, Battacharya , David, Cowburn , Ranajeet, Ghose
Measurement of steady-state 15N-{1H} nuclear Overhauser effects forms a cornerstone of most methods to determine protein backbone dynamics from spin-relaxation data, since it is the most reliable probe of very fast motions on the ps-ns timescale. We have, in two previous publications (J. Magn. Reson. 192 (2008), 302-313; J. Am. Chem. Soc. 131 (2009), 6048-6049) reevaluated spin-dynamics during steady-state (or “saturated”) and reference experiments, both of which are required to determine the NOE ratio. Here we assess the performance of several windowed and windowless sequences to achieve effective saturation of protons in steady-state experiments. We also evaluate the influence of...
[NMR paper] Effects of remote mutation on the autolysis of HIV-1 PR: X-ray and NMR investigations
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Biochem Biophys Res Commun. 2002 Jun 7;294(2):395-401
Authors: Kumar M, Kannan KK, Hosur MV, Bhavesh NS, Chatterjee A, Mittal R, Hosur RV
Autolysis rates of the C95M and C95M/C1095A mutants of a HIV-1 protease tethered dimer have been determined by real time NMR and it is observed that the double mutant has approximately two times higher rate. X-ray...
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[NMR paper] Val(659)-->Glu mutation within the transmembrane domain of ErbB-2: effects measured b
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Biochemistry. 2000 May 30;39(21):6572-80
Authors: Sharpe S, Barber KR, Grant CW
Certain point mutations within the hydrophobic transmembrane domains of class I receptor tyrosine kinases have been associated with oncogenic transformation in vitro and in vivo . An important...
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[NMR paper] The effects of proteins on [Ca2+] measurement: different effects on fluorescent and N
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Cell Calcium. 1996 Nov;20(5):425-30
Authors: Matsuda S, Kusuoka H, Hashimoto K, Tsujimura E, Nishimura T
Previous reports showed that the presence of proteins shifts the apparent dissociation constant (Kd) of a fluorescent dye indicator to...
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Biochemistry. 1993 Jan 19;32(2):657-62
Authors: Matthews SJ, Jandu SK, Leatherbarrow RJ
Recombinant chymotrypsin inhibitor 2 (CI-2) and the three mutants Ile39-->Val, Ile39-->Leu, and Arg67-->Ala were successfully enriched with tryptophan at position...
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[NMR paper] Structural effects of hydration: studies of lysozyme by 13C solids NMR.
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Biopolymers. 1990 Dec;29(14):1801-6
Authors: Kennedy SD, Bryant RG
13C-nmr spectra of lysozyme obtained at 50.3 MHz using both static and magic-angle-spinning-cross-polarization methods are reported at several water contents. The line widths and consequent resolution in the hydrated material is substantially improved over that in the lyophilized protein. The line narrowing is not...
On the measurement of 15N-{1H} nuclear Overhauser effects. 2. Effects of the saturati
Linear Mode
