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Default Mapping Functional Interaction Sites of Human Prune C-Terminal Domain by NMR Spectroscopy in Human Cell Lysates.

Mapping Functional Interaction Sites of Human Prune C-Terminal Domain by NMR Spectroscopy in Human Cell Lysates.

Mapping Functional Interaction Sites of Human Prune C-Terminal Domain by NMR Spectroscopy in Human Cell Lysates.

Chemistry. 2013 Aug 12;

Authors: Diana D, Smaldone G, De Antonellis P, Pirone L, Carotenuto M, Alonzi A, Di Gaetano S, Zollo M, Pedone EM, Fattorusso R

Abstract
Get well prune: The C-terminal third domain of h-prune is largely unfolded and involved in relevant protein-protein interactions, particularly with Nm23-H1, GSK-3? and gelsolin. This study shows that protein functions mediated by protein-protein interactions can be accurately followed in cell lysates by using NMR spectroscopy fast experiments, which could be easily used for a very efficient NMR drug discovery strategy.


PMID: 23939913 [PubMed - as supplied by publisher]



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