Macromolecular NMR spectroscopy for the non-spectroscopist: beyond macromolecular solution structure determination.
FEBS J. 2011 Jan 7;
Authors: Bieri M, Kwan AH, Mobli M, King GF, Mackay JP, Gooley PR
A strength of NMR spectroscopy is its ability to monitor, on an atomic level, molecular changes and interactions. In this article, which is intended for non-spectroscopists, we describe major uses of NMR in protein science beyond solution structure determination. After first touching on how NMR can be used to quickly determine whether a mutation induces structural perturbations in a protein, we describe the unparalleled ability of NMR to monitor binding interactions over a wide range of affinities, molecular masses and solution conditions. We discuss the use of NMR to measure the dynamics of proteins at the atomic level and over a wide range of timescales. Finally, we outline new and expanding areas such as macromolecular structure determination in multi-component systems as well as in the solid state and in vivo.
PMID: 21214861 [PubMed - as supplied by publisher]
Macromolecular NMR spectroscopy for the non-spectroscopist.
Macromolecular NMR spectroscopy for the non-spectroscopist.
Macromolecular NMR spectroscopy for the non-spectroscopist.
FEBS J. 2011 Mar;278(5):687-703
Authors: Kwan AH, Mobli M, Gooley PR, King GF, Mackay JP
NMR spectroscopy is a powerful tool for studying the structure, function and dynamics of biological macromolecules. However, non-spectroscopists often find NMR theory daunting and data interpretation nontrivial. As the first of two back-to-back reviews on NMR spectroscopy aimed at non-spectroscopists, the present review first provides an...
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04-20-2011 07:15 PM
postdoctoral position in macromolecular NMR
postdoctoral position in macromolecular NMR
There is an opening for a postdoctoral position in macromolecular NMR
and structural biology at the Structural Biophysics Laboratory, National
Cancer Institute, Frederick, MD.
A highly motivated individual is sought to conduct studies in NMR, dynamics,
and integrated structural and functional understanding of proteins and complexes
in the ubiquitination pathway. For an example of the type of work please see
Mol. Cell 34(6):674 . Individuals interested in spectroscopy, new methods, and/or
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08-21-2010 05:17 AM
Macromolecular NMR Spectroscopist
Macromolecular NMR Spectroscopist
The Structural Biophysics Laboratory, National Cancer Institute is seeking the support of a Macromolecular NMR spectroscopist who will work with and support the PIs in the Structural Biophysics Laboratory (SBL) For info on the SBL visit: http://go.cancer.gov/sbljobs]. The position will be hired by the SAIC-Frederick and dedicated to the SBL.
The Scientist II will work with and support principal investigators in the Structural Biophysics Laboratory (SBL) in the determination of biological macromolecular structures using high-field NMR (500-850 MHz...
nmrlearner
Job marketplace
0
08-21-2010 05:17 AM
Macromolecular NMR Spectroscopist
Macromolecular NMR Spectroscopist
The Structural Biophysics Laboratory, National Cancer Institute is seeking the support of a Macromolecular NMR spectroscopist who will work with and support the PIs in the Structural Biophysics Laboratory (SBL) For info on the SBL visit: http://go.cancer.gov/sbljobs]. The position will be hired by the SAIC-Frederick and dedicated to the SBL.
The Scientist II will work with and support principal investigators in the Structural Biophysics Laboratory (SBL) in the determination of biological macromolecular structures using high-field NMR (500-850 MHz...
Macromolecular NMR spectroscopy for the non-spectroscopist: beyond macromolecular solution structure determination.
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