Macromolecular NMR spectroscopy for the non-spectroscopist.

		BioNMR > Educational resources > Journal club
Macromolecular NMR spectroscopy for the non-spectroscopist.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

View First Unread

Thread Tools

Search this Thread

Rating:

Display Modes

04-20-2011, 07:15 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,174

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Macromolecular NMR spectroscopy for the non-spectroscopist.

Macromolecular NMR spectroscopy for the non-spectroscopist.

Macromolecular NMR spectroscopy for the non-spectroscopist.

FEBS J. 2011 Mar;278(5):687-703

Authors: Kwan AH, Mobli M, Gooley PR, King GF, Mackay JP

NMR spectroscopy is a powerful tool for studying the structure, function and dynamics of biological macromolecules. However, non-spectroscopists often find NMR theory daunting and data interpretation nontrivial. As the first of two back-to-back reviews on NMR spectroscopy aimed at non-spectroscopists, the present review first provides an introduction to the basics of macromolecular NMR spectroscopy, including a discussion of typical sample requirements and what information can be obtained from simple NMR experiments. We then review the use of NMR spectroscopy for determining the 3D structures of macromolecules and examine how to judge the quality of NMR-derived structures.

PMID: 21214860 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Macromolecular NMR spectroscopy for the non-spectroscopist: beyond macromolecular solution structure determination. Macromolecular NMR spectroscopy for the non-spectroscopist: beyond macromolecular solution structure determination. Macromolecular NMR spectroscopy for the non-spectroscopist: beyond macromolecular solution structure determination. FEBS J. 2011 Jan 7; Authors: Bieri M, Kwan AH, Mobli M, King GF, Mackay JP, Gooley PR A strength of NMR spectroscopy is its ability to monitor, on an atomic level, molecular changes and interactions. In this article, which is intended for non-spectroscopists, we describe major uses of NMR in protein science beyond...	nmrlearner	Journal club	0	01-11-2011 11:27 PM
Positions in Biomolecular NMR Spectroscopy:senior NMR spectroscopist, postdoctoral as Positions in Biomolecular NMR Spectroscopy:senior NMR spectroscopist, postdoctoral associate, techni - CARIM school for Cardiovascular Diseases - Maastricht, Netherlands Positions in Biomolecular NMR Spectroscopy at Maastricht Description The Maastricht University Medical Center and the Faculty of Health, Medicine and Life Sciences (FHML) seek to fill several positions for the newly established center for NMR Structural Biology within the CARIM School for Cardiovascular Diseases and the Maastricht-Centre... More...	nmrlearner	Job marketplace	0	09-21-2010 06:43 PM
Macromolecular NMR Spectroscopist Macromolecular NMR Spectroscopist The Structural Biophysics Laboratory, National Cancer Institute is seeking the support of a Macromolecular NMR spectroscopist who will work with and support the PIs in the Structural Biophysics Laboratory (SBL) For info on the SBL visit: http://go.cancer.gov/sbljobs]. The position will be hired by the SAIC-Frederick and dedicated to the SBL. The Scientist II will work with and support principal investigators in the Structural Biophysics Laboratory (SBL) in the determination of biological macromolecular structures using high-field NMR (500-850 MHz...	nmrlearner	Job marketplace	0	08-21-2010 05:17 AM
postdoctoral position in macromolecular NMR postdoctoral position in macromolecular NMR There is an opening for a postdoctoral position in macromolecular NMR and structural biology at the Structural Biophysics Laboratory, National Cancer Institute, Frederick, MD. A highly motivated individual is sought to conduct studies in NMR, dynamics, and integrated structural and functional understanding of proteins and complexes in the ubiquitination pathway. For an example of the type of work please see Mol. Cell 34(6):674 . Individuals interested in spectroscopy, new methods, and/or	nmrlearner	Job marketplace	0	08-21-2010 05:14 AM
Macromolecular NMR Spectroscopist Macromolecular NMR Spectroscopist The Structural Biophysics Laboratory, National Cancer Institute is seeking the support of a Macromolecular NMR spectroscopist who will work with and support the PIs in the Structural Biophysics Laboratory (SBL) For info on the SBL visit: http://go.cancer.gov/sbljobs]. The position will be hired by the SAIC-Frederick and dedicated to the SBL. The Scientist II will work with and support principal investigators in the Structural Biophysics Laboratory (SBL) in the determination of biological macromolecular structures using high-field NMR (500-850 MHz...	nmrlearner	Job marketplace	0	08-21-2010 05:14 AM
Quality of Macromolecular Models Quality of Macromolecular Models More...	nmrlearner	NMR bookmarks	0	08-19-2010 02:34 PM
Tim Logan's lecutre notes for Macromolecular NMR Spectroscopy class <CENTER> http://www.sb.fsu.edu/~logan/index_files/fkbp.GIF Tim Logan's group website	nmrlearner	Educational web pages	1	03-31-2007 12:08 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off