Abstract
Ever since scientists realized that cells are the basic building blocks of all life, they have been developing tools to look inside them to reveal the architectures and mechanisms that define their biological functions. Whereas "looking into cells" is typically said in reference to optical microscopy, high-resolution in-cell and on-cell nuclear magnetic resonance (NMR) spectroscopy is a powerful method that offers exciting new possibilities for structural and functional studies in and on live cells. In contrast to conventional imaging techniques, in- and on-cell NMR methods do not provide spatial information on cellular biomolecules. Instead, they enable atomic-resolution insights into the native cell states of proteins, nucleic acids, glycans, and lipids. Here we review recent advances and developments in both fields and discuss emerging concepts that have been delineated with these methods.
PMID: 24895852 [PubMed - as supplied by publisher]
[NMR paper] Atomic-resolution monitoring of protein maturation in live human cells by NMR.
Atomic-resolution monitoring of protein maturation in live human cells by NMR.
Atomic-resolution monitoring of protein maturation in live human cells by NMR.
Nat Chem Biol. 2013 Mar 3;
Authors: Banci L, Barbieri L, Bertini I, Luchinat E, Secci E, Zhao Y, Aricescu AR
Abstract
We use NMR directly in live human cells to describe the complete post-translational maturation process of human superoxide dismutase 1 (SOD1). We follow, at atomic resolution, zinc binding, homodimer formation and copper uptake, and discover that copper chaperone for...
nmrlearner
Journal club
0
03-05-2013 03:25 PM
[NMR paper] Quantitative comparison of protein dynamics in live cells and in vitro by in-cell (19)F-NMR.
Quantitative comparison of protein dynamics in live cells and in vitro by in-cell (19)F-NMR.
Quantitative comparison of protein dynamics in live cells and in vitro by in-cell (19)F-NMR.
Chem Commun (Camb). 2013 Feb 26;
Authors: Takaoka Y, Kioi Y, Morito A, Otani J, Arita K, Ashihara E, Ariyoshi M, Tochio H, Shirakawa M, Hamachi I
Abstract
Here we describe how a (19)F-probe incorporated into an endogenous protein by a chemical biology method revealed protein dynamics. By explicit determination of ligand-bound and unbound structures with...
nmrlearner
Journal club
0
02-27-2013 06:47 PM
In-cell NMR spectroscopy
In-cell NMR spectroscopy
October 2011
Publication year: 2011
Source:Progress in Nuclear Magnetic Resonance Spectroscopy, Volume 59, Issue 3</br>
</br>
</br>
</br></br>
nmrlearner
Journal club
0
12-01-2012 06:10 PM
In-cell NMR spectroscopy
In-cell NMR spectroscopy
Publication year: 2011
Source:Progress in Nuclear Magnetic Resonance Spectroscopy, Volume 59, Issue 3</br>
Andres Y. Maldonado, David S. Burz, Alexander Shekhtman</br>
</br>
</br></br>
[NMR paper] What NMR can tell us about where lung surfactant proteins live.
What NMR can tell us about where lung surfactant proteins live.
Related Articles What NMR can tell us about where lung surfactant proteins live.
Biochem Soc Trans. 1997 Aug;25(3):1103-7
Authors: Morrow MR, Taneva S, Dico AS, Hancock J, Keough KM
2H-NMR is beginning to provide some insights into the way in which the hydrophobic surfactant proteins SP-B and SP-C interact with phospholipid bilayers in multilamellar structures. Both proteins have a significant effect on slow bilayer motions. In many ways, the effect of SP-C on the surrounding...
Live Cell NMR.
Linear Mode
