Related ArticlesLimitations to Starch Utilization in Barramundi (Lates calcarifer) as Revealed by NMR-Based Metabolomics.
Front Physiol. 2020;11:205
Authors: Palma M, Trenkner LH, Rito J, Tavares LC, Silva E, Glencross BD, Jones JG, Wade NM, Viegas I
Abstract
Practical diets for commercial barramundi production rarely contain greater than 10% starch, used mainly as a binding agent during extrusion. Alternative ingredients such as digestible starch have shown some capacity to spare dietary protein catabolism to generate glucose. In the present study, a carnivorous fish species, the Asian seabass (Lates calcarifer) was subjected to two diets with the same digestible energy: Protein (P) - with high protein content (no digestible starch); and Starch (S) - with high digestible (pregelatinized) starch content. The effects of a high starch content diet on hepatic glycogen synthesis as well as the muscle and liver metabolome were studied using a complementary approach of 1H and 2H NMR. The hepatosomatic index was lower for fish fed high starch content diet while the concentration of hepatic glycogen was similar between groups. However, increased glycogen synthesis via the direct pathway was observed in the fish fed high starch content diet which is indicative of increased carbohydrate utilization. Multivariate analysis also showed differences between groups in the metabolome of both tissues. Univariate analysis revealed more variations in liver than in muscle of fish fed high starch content diet. Variations in metabolome were generally in agreement with the increase in the glycogen synthesis through direct pathway, however, this metabolic shift seemed to be insufficient to keep the growth rate as ensured by the diet with high protein content. Although liver glycogen does not make up a substantial quantity of total stored dietary energy in carnivorous fish, it is a key regulatory intermediate in dietary energy utilization.
[NMR paper] NMR-Based Metabolomics of Oral Biofluids.
NMR-Based Metabolomics of Oral Biofluids.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles NMR-Based Metabolomics of Oral Biofluids.
Methods Mol Biol. 2017;1537:79-105
Authors: Schirra HJ, Ford PJ
Abstract
NMR-based metabolomics is an established technique for characterizing the metabolite profile of biological fluids and investigating how metabolite profiles change in response to biological and/or clinical stimuli. Thus,...
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[NMR paper] Toxic responses of Perna viridis hepatopancreas exposed to DDT, benzo(a)pyrene and their mixture uncovered by iTRAQ-based proteomics and NMR-based metabolomics.
Toxic responses of Perna viridis hepatopancreas exposed to DDT, benzo(a)pyrene and their mixture uncovered by iTRAQ-based proteomics and NMR-based metabolomics.
Toxic responses of Perna viridis hepatopancreas exposed to DDT, benzo(a)pyrene and their mixture uncovered by iTRAQ-based proteomics and NMR-based metabolomics.
Aquat Toxicol. 2017 Sep 11;192:48-57
Authors: Song Q, Zhou H, Han Q, Diao X
Abstract
Dichlorodiphenyltrichloroethane (DDT) and benzo(a)pyrene (BaP) are environmental estrogens (EEs) that are ubiquitous in the...
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[NMR paper] Biochemical characterization and (1)H NMR based metabolomics revealed Melicope lunu-ankenda leaf extract a potent anti-diabetic agent in rats.
Biochemical characterization and (1)H NMR based metabolomics revealed Melicope lunu-ankenda leaf extract a potent anti-diabetic agent in rats.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:--www.biomedcentral.com-graphics-pubmed-BioMedCentral_free_1.png http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:--www.ncbi.nlm.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Biochemical characterization and (1)H NMR based metabolomics revealed Melicope lunu-ankenda leaf extract a potent anti-diabetic agent in rats.
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[NMR paper] Protein catabolism and high lipid metabolism associated with long-distance exercise are revealed by plasma NMR metabolomics in endurance horses.
Protein catabolism and high lipid metabolism associated with long-distance exercise are revealed by plasma NMR metabolomics in endurance horses.
Related Articles Protein catabolism and high lipid metabolism associated with long-distance exercise are revealed by plasma NMR metabolomics in endurance horses.
PLoS One. 2014;9(3):e90730
Authors: Le Moyec L, Robert C, Triba MN, Billat VL, Mata X, Schibler L, Barrey E
Abstract
During long distance endurance races, horses undergo high physiological and metabolic stresses. The adaptation processes...
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Formulation and utilization of choline based samples for dissolution dynamic nuclear polarization
From The DNP-NMR Blog:
Formulation and utilization of choline based samples for dissolution dynamic nuclear polarization
Bowen, S. and J.H. Ardenkjaer-Larsen, Formulation and utilization of choline based samples for dissolution dynamic nuclear polarization. J Magn Reson, 2013. 236(0): p. 26-30.
http://www.ncbi.nlm.nih.gov/pubmed/24036470
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12-02-2013 11:48 PM
[NMR paper] Analysis of Proteinogenic Amino Acid and Starch Labeling by 2D NMR.
Analysis of Proteinogenic Amino Acid and Starch Labeling by 2D NMR.
Analysis of Proteinogenic Amino Acid and Starch Labeling by 2D NMR.
Methods Mol Biol. 2014;1090:87-105
Authors: Truong Q, Shanks JV
Abstract
Comprehensive analysis of isotopic labeling patterns of metabolites in proteinogenic amino acids and starch for plant systems lay in the powerful tool of 2-Dimensional Nuclear Magnetic Resonance (2D NMR) spectroscopy. From (13)C-labeling experiments, 2D NMR provides information on the labeling of particular carbon positions, which...
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11-14-2013 11:12 AM
Nuclear Magnetic Resonance (NMR)-Based Metabolomics.
Nuclear Magnetic Resonance (NMR)-Based Metabolomics.
Nuclear Magnetic Resonance (NMR)-Based Metabolomics.
Methods Mol Biol. 2011;708:321-34
Authors: Keun HC, Athersuch TJ
Biofluids are by far the most commonly studied sample type in metabolic profiling studies, encompassing blood, urine, cerebrospinal fluid, cell culture media and many others. A number of these fluids can be obtained at a high sampling frequency with minimal invasion, permitting detailed characterisation of dynamic metabolic events. One of the attractive properties of...
Limitations to Starch Utilization in Barramundi (Lates calcarifer) as Revealed by NMR-Based Metabolomics.
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