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Default A Ligand-Based NMR Screening Approach for the Identification and Characterization of Inhibitors and Promoters of Amyloid Peptide Aggregation.

A Ligand-Based NMR Screening Approach for the Identification and Characterization of Inhibitors and Promoters of Amyloid Peptide Aggregation.

Related Articles A Ligand-Based NMR Screening Approach for the Identification and Characterization of Inhibitors and Promoters of Amyloid Peptide Aggregation.

ChemMedChem. 2017 Sep 07;12(17):1458-1463

Authors: Dalvit C, Santi S, Neier R

Abstract
Over the years a significant amount of effort has been put into the development of rapid and reliable methods to monitor the aggregation dynamics of the ?1-42 amyloid peptide in real time. We present an alternative approach based on a suitable reporter or spy molecule and three different NMR experiments: WaterLOGSY, (1) H selective T1 filter, and (19) F T2 filter, for monitoring the initial self-aggregation process kinetics of the ?1-42 amyloid peptide and identifying molecules that retard or accelerate the self-aggregation process. Although the proposed method is not a high-throughput assay, it avoids problems associated with interference events that are sometimes observed in fluorescence-based assays.


PMID: 28722296 [PubMed - indexed for MEDLINE]



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