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Default LEGO-NMR Spectroscopy: A Method to Visualize Individual Subunits in Large Heteromeric Complexes.

LEGO-NMR Spectroscopy: A Method to Visualize Individual Subunits in Large Heteromeric Complexes.

Related Articles LEGO-NMR Spectroscopy: A Method to Visualize Individual Subunits in Large Heteromeric Complexes.

Angew Chem Int Ed Engl. 2013 Aug 14;

Authors: Mund M, Overbeck JH, Ullmann J, Sprangers R

Abstract
Seeing the big picture: Asymmetric macromolecular complexes that are NMR active in only a subset of their subunits can be prepared, thus decreasing NMR spectral complexity. For the hetero heptameric LSm1-7 and LSm2-8 rings NMR spectra of the individual subunits of the complete complex are obtained, showing a conserved RNA binding site. This LEGO-NMR technique makes large asymmetric complexes accessible to detailed NMR spectroscopic studies.


PMID: 23946163 [PubMed - as supplied by publisher]



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