We describe a novel, laser-initiated radical trifluoromethylation for protein footprinting and establish its broad residue coverage. *CF3 reacts with 18 of 20 common amino acids including Gly, Ala, Ser, Thr, Asp, Glu that are relatively "silent" with *OH. This new approach to footprinting is a bridge between trifluoromethylation in materials and medicinal chemistry and structural biology and biotechnology. Its application to a membrane protein and to myoglobin show that the approach is sensitive to protein conformational change and solvent accessibility.
Quantifying Protection in Disordered Proteins UsingMillisecond Hydrogen Exchange-Mass Spectrometry and Peptic ReferencePeptides
Quantifying Protection in Disordered Proteins UsingMillisecond Hydrogen Exchange-Mass Spectrometry and Peptic ReferencePeptides
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Biochemistry
DOI: 10.1021/acs.biochem.6b01312
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Structural Analysis of the Glycosylated Intact HIV-1 gp120–b12 Antibody Complex Using Hydroxyl Radical Protein Footprinting
Structural Analysis of the Glycosylated Intact HIV-1 gp120–b12 Antibody Complex Using Hydroxyl Radical Protein Footprinting
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Biochemistry
DOI: 10.1021/acs.biochem.6b00888
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[NMR paper] Native mass spectrometry of photosynthetic pigment-protein complexes.
Native mass spectrometry of photosynthetic pigment-protein complexes.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Native mass spectrometry of photosynthetic pigment-protein complexes.
FEBS Lett. 2013 Jan 18;
Authors: Zhang H, Cui W, Gross ML, Blankenship RE
Abstract
Native mass spectrometry (MS), or as is sometimes called "native electrospray ionization" allows proteins in their native or near-native states in solution to be introduced into the gas phase and...
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[NMR paper] NMR and mass spectrometry studies of putative interactions of cell cycle proteins pRb
NMR and mass spectrometry studies of putative interactions of cell cycle proteins pRb and CDK6 with cell differentiation proteins MyoD and ID-2.
Related Articles NMR and mass spectrometry studies of putative interactions of cell cycle proteins pRb and CDK6 with cell differentiation proteins MyoD and ID-2.
Biochim Biophys Acta. 2005 Jun 15;1750(1):48-60
Authors: Smialowski P, Singh M, Mikolajka A, Majumdar S, Joy JK, Nalabothula N, Krajewski M, Degenkolbe R, Bernard HU, Holak TA
Cell growth and differentiation require precise coordination of...
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[NMR paper] Mass spectrometry assisted assignment of NMR resonances in 15N labeled proteins.
Mass spectrometry assisted assignment of NMR resonances in 15N labeled proteins.
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J Am Chem Soc. 2004 Nov 10;126(44):14377-9
Authors: Feng L, Orlando R, Prestegard JH
Application of nuclear magnetic resonance (NMR) methods for the structural characterization to larger and more complex protein systems can be facilitated through the development of new methods for resonance assignment. Here, a novel approach that relies on integration of NMR and mass...
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[NMR paper] Mass spectrometry--a useful tool for the protein X-ray crystallographer and NMR spect
Mass spectrometry--a useful tool for the protein X-ray crystallographer and NMR spectroscopist.
Related Articles Mass spectrometry--a useful tool for the protein X-ray crystallographer and NMR spectroscopist.
Structure. 1994 Jun 15;2(6):465-7
Authors: Chait BT
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[NMR paper] Mass spectrometry--a useful tool for the protein X-ray crystallographer and NMR spect
Mass spectrometry--a useful tool for the protein X-ray crystallographer and NMR spectroscopist.
Related Articles Mass spectrometry--a useful tool for the protein X-ray crystallographer and NMR spectroscopist.
Structure. 1994 Jun 15;2(6):465-7
Authors: Chait BT
Laser-initiated Radical Trifluoromethylation of Peptides and Proteins and Its Application to Mass Spectrometry-Based Protein Footprinting
Linear Mode
