A large geometric distortion in the first photointermediate of rhodopsin, determined by double-quantum solid-state NMR
A large geometric distortion in the first photointermediate of rhodopsin, determined by double-quantum solid-state NMR
Abstract Double-quantum magic-angle-spinning NMR experiments were performed on 11,12-13C2-retinylidene-rhodopsin under illumination at low temperature, in order to characterize torsional angle changes at the C11-C12 photoisomerization site. The sample was illuminated in the NMR rotor at low temperature (~120 K) in order to trap the primary photointermediate, bathorhodopsin. The NMR data are consistent with a strong torsional twist of the HCCH moiety at the isomerization site. Although the HCCH torsional twist was determined to be at least 40°, it was not possible to quantify it more closely. The presence of a strong twist is in agreement with previous Raman observations. The energetic implications of this geometric distortion are discussed.
Source: Journal of Biomolecular NMR |
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