Related ArticlesLactose in human breast milk an inducer of innate immunity with implications for a role in intestinal homeostasis.
PLoS One. 2013;8(1):e53876
Authors: Cederlund A, Kai-Larsen Y, Printz G, Yoshio H, Alvelius G, Lagercrantz H, Strömberg R, Jörnvall H, Gudmundsson GH, Agerberth B
Abstract
Postpartum, infants have not yet established a fully functional adaptive immune system and are at risk of acquiring infections. Hence, newborns are dependent on the innate immune system with its antimicrobial peptides (AMPs) and proteins expressed at epithelial surfaces. Several factors in breast milk are known to confer immune protection, but which the decisive factors are and through which manner they work is unknown. Here, we isolated an AMP-inducing factor from human milk and identified it by electrospray mass spectrometry and NMR to be lactose. It induces the gene (CAMP) that encodes the only human cathelicidin LL-37 in colonic epithelial cells in a dose- and time-dependent manner. The induction was suppressed by two different p38 antagonists, indicating an effect via the p38-dependent pathway. Lactose also induced CAMP in the colonic epithelial cell line T84 and in THP-1 monocytes and macrophages. It further exhibited a synergistic effect with butyrate and phenylbutyrate on CAMP induction. Together, these results suggest an additional function of lactose in innate immunity by upregulating gastrointestinal AMPs that may lead to protection of the neonatal gut against pathogens and regulation of the microbiota of the infant.
[NMR paper] CD and NMR studies of prion protein (PrP) helix 1. Novel implications for its role in
CD and NMR studies of prion protein (PrP) helix 1. Novel implications for its role in the PrPC-->PrPSc conversion process.
Related Articles CD and NMR studies of prion protein (PrP) helix 1. Novel implications for its role in the PrPC-->PrPSc conversion process.
J Biol Chem. 2003 Dec 12;278(50):50175-81
Authors: Ziegler J, Sticht H, Marx UC, Müller W, Rösch P, Schwarzinger S
The conversion of prion helix 1 from an alpha-helical into an extended conformation is generally assumed to be an essential step in the conversion of the cellular isoform...
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[NMR paper] NMR structure of the sterol carrier protein-2: implications for the biological role.
NMR structure of the sterol carrier protein-2: implications for the biological role.
Related Articles NMR structure of the sterol carrier protein-2: implications for the biological role.
J Mol Biol. 2000 Jan 21;295(3):595-603
Authors: García FL, Szyperski T, Dyer JH, Choinowski T, Seedorf U, Hauser H, Wüthrich K
The determination of the NMR structure of the sterol carrier protein-2 (SCP2), analysis of backbone (15)N spin relaxation parameters and NMR studies of nitroxide spin-labeled substrate binding are presented as a new basis for...
[NMR paper] Kinetics of choline transport and phosphorylation in human breast cancer cells; NMR a
Kinetics of choline transport and phosphorylation in human breast cancer cells; NMR application of the zero trans method.
Related Articles Kinetics of choline transport and phosphorylation in human breast cancer cells; NMR application of the zero trans method.
Anticancer Res. 1996 May-Jun;16(3B):1375-80
Authors: Katz-Brull R, Degani H
The mechanism and kinetics of choline transport and phosphorylation in MCF7 human breast cancer cells was studied by 31P, 13C and 2H NMR, applying the zero trans method. Choline was transported by a...
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NMR structure of the calponin homology domain of human IQGAP1 and its implications fo
NMR structure of the calponin homology domain of human IQGAP1 and its implications for the actin recognition mode
Content Type Journal Article
DOI 10.1007/s10858-010-9434-8
Authors
Ryo Umemoto, The University of Tokyo Graduate School of Pharmaceutical Sciences Hongo, Bunkyo-ku Tokyo 113-0033 Japan
Noritaka Nishida, The University of Tokyo Graduate School of Pharmaceutical Sciences Hongo, Bunkyo-ku Tokyo 113-0033 Japan
Shinji Ogino, The University of Tokyo Graduate School of Pharmaceutical Sciences Hongo, Bunkyo-ku Tokyo 113-0033 Japan
Lactose in human breast milk an inducer of innate immunity with implications for a role in intestinal homeostasis.
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