Abstract A blood pre-centrifugation delay of 24 h at room temperature influenced the proton NMR spectroscopic profiles of human serum. A blood pre-centrifugation delay of 24 h at 4°C did not influence the spectroscopic profile as compared with 4 h delays at either room temperature or 4°C. Five or ten serum freezeâ??thaw cycles also influenced the proton NMR spectroscopic profiles. Certain common in vitro preanalytical variations occurring in biobanks may impact the metabolic profile of human serum.
Mutations in the Saccharomyces cerevisiae succinate dehydrogenase result in distinct metabolic phenotypes revealed through (1)H NMR-based metabolic footprinting.
Mutations in the Saccharomyces cerevisiae succinate dehydrogenase result in distinct metabolic phenotypes revealed through (1)H NMR-based metabolic footprinting.
Mutations in the Saccharomyces cerevisiae succinate dehydrogenase result in distinct metabolic phenotypes revealed through (1)H NMR-based metabolic footprinting.
J Proteome Res. 2010 Dec 3;9(12):6729-39
Authors: Szeto SS, Reinke SN, Sykes BD, Lemire BD
Metabolomics is a powerful method of examining the intricate connections between mutations, metabolism, and disease. Metabolic...
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Changes in the NMR metabolic profile of human microglial cells exposed to lipopolysaccharide or morphine.
Changes in the NMR metabolic profile of human microglial cells exposed to lipopolysaccharide or morphine.
Changes in the NMR metabolic profile of human microglial cells exposed to lipopolysaccharide or morphine.
J Neuroimmune Pharmacol. 2010 Dec;5(4):574-81
Authors: El Ghazi I, Sheng WS, Hu S, Reilly BG, Lokensgard JR, Rock RB, Peterson PK, Wilcox GL, Armitage IM
Microglial cells play a major role in host defense of the central nervous system. Once activated, several functional properties are up-regulated including migration, phagocytosis, and...
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03-01-2011 12:14 PM
A new method for the determination of free L: -carnitine in serum samples based on high field single quantum coherence filtering (1)H-NMR spectroscopy.
A new method for the determination of free L: -carnitine in serum samples based on high field single quantum coherence filtering (1)H-NMR spectroscopy.
A new method for the determination of free L: -carnitine in serum samples based on high field single quantum coherence filtering (1)H-NMR spectroscopy.
Anal Bioanal Chem. 2011 Jan 11;
Authors: Tsiafoulis CG, Exarchou V, Tziova PP, Bairaktari E, Gerothanassis IP, Troganis AN
The rapid and accurate determination of specific metabolites present in biofluids is a very demanding task which is essential...
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The impact of prenatal disorders on the metabolic profile of 2nd trimester amniotic f
THE IMPACT OF PRENATAL DISORDERS ON THE METABOLIC PROFILE OF 2ND TRIMESTER AMNIOTIC FLUID: A NUCLEAR MAGNETIC RESONANCE (NMR) METABONOMIC STUDY.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles THE IMPACT OF PRENATAL DISORDERS ON THE METABOLIC PROFILE OF 2ND TRIMESTER AMNIOTIC FLUID: A NUCLEAR MAGNETIC RESONANCE (NMR) METABONOMIC STUDY.
J Proteome Res. 2010 Sep 17;
Authors: Graça G, Duarte IF, Barros AS, Goodfellow BJ, Diaz S, Pinto J, Carreira I, Galhano E, Pita C, Gil AM
This paper...
[NMR paper] The influence of hydration on the conformation of bovine serum albumin studied by sol
The influence of hydration on the conformation of bovine serum albumin studied by solid-state 13C-NMR spectroscopy.
Related Articles The influence of hydration on the conformation of bovine serum albumin studied by solid-state 13C-NMR spectroscopy.
Biopolymers. 1993 Dec;33(12):1871-6
Authors: Gregory RB, Gangoda M, Gilpin RK, Su W
13C proton-decoupled cross-polarization magic-angle spinning nmr spectra of bovine serum albumin are reported as a function of hydration. Increases in hydration level enhance the resolution of the peak centered at...
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[NMR paper] Volatile anesthetics compete for common binding sites on bovine serum albumin: a 19F-
Volatile anesthetics compete for common binding sites on bovine serum albumin: a 19F-NMR study.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Volatile anesthetics compete for common binding sites on bovine serum albumin: a 19F-NMR study.
Proc Natl Acad Sci U S A. 1993 Jul 15;90(14):6478-82
Authors: Dubois BW, Cherian SF, Evers AS
There is controversy as to the molecular nature of volatile anesthetic target sites. One proposal is that volatile anesthetics...
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08-22-2010 03:01 AM
Major groove width variations in RNA structures determined by NMR and impact of 13C r
Abstract Ribonucleic acid structure determination by NMR spectroscopy relies primarily on local structural restraints provided by 1Hâ?? 1H NOEs and J-couplings. When employed loosely, these restraints are broadly compatible with A- and B-like helical geometries and give rise to calculated structures that are highly sensitive to the force fields employed during refinement. A survey of recently reported NMR structures reveals significant variations in helical parameters, particularly the major groove width. Although helical parameters observed in high-resolution X-ray crystal structures of...
Influence of common preanalytical variations on the metabolic profile of serum samples in biobanks
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