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Default Improved excitation pulse bandwidths using shaped pulses, with application to heteron

Improved excitation pulse bandwidths using shaped pulses, with application to heteronuclear half filters in macromolecular NMR.

Related Articles Improved excitation pulse bandwidths using shaped pulses, with application to heteronuclear half filters in macromolecular NMR.

J Magn Reson B. 1995 Jul;108(1):12-21

Authors: Hyre DE, Spicer LD

The advantageous use of sinc-shaped pulses in heteronuclear half filters is explored for studying biological macromolecules. The typical square, or hard, pulse used in half-filter pulse sequences for heteronuclear excitation results in suboptimal suppression of unwanted resonances due to incomplete inversion of spins. The novel use of short-duration shaped pulses applied at high power achieves more uniform excitation profiles over the extended frequency ranges often needed for heteronuclear filtering. This approach is used in the development of a double-tuned omega 1, omega 2-double-half-filtered, double-quantum-filtered COSY experiment. The efficiency of this experiment incorporating sinc pulses compares favorably with that obtained with square pulses in a mixture of 13C-labeled and unlabeled amino acids. Sinc-pulse-filtered spectra of the 24 kDa methionine repressor protein dimer MetJ, uniformly 13C-labeled expect at two unlabeled methionine residues, were also obtained to demonstrate the utility of this approach in biomacromolecular studies.

PMID: 7627432 [PubMed - indexed for MEDLINE]



Source: PubMed
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