Publication date: Available online 18 March 2017 Source:Solid State Nuclear Magnetic Resonance
Author(s): Joćo Medeiros-Silva, Shehrazade Jekhmane, Marc Baldus, Markus Weingarth
1H-detected solid-state NMR in combination with 1H/2D exchange steps allows for the direct identification of very strong hydrogen bonds in membrane proteins. On the example of the membrane-embedded potassium channel KcsA, we quantify the longevity of such very strong hydrogen bonds by combining time-resolved 1H-detected solid-state NMR and molecular dynamics simulations. In particular, we show that the carboxyl-side chain of the highly conserved residue Glu51 is involved in ultra-strong hydrogen bonds, which are fully-water-exposed and yet stable for weeks. The astonishing stability of these hydrogen bonds is important for the structural integrity of potassium channels, which we further corroborate by computational studies. Graphical abstract
HighField Solid-State NMR Spectroscopy Investigation of 15N-Labeled Rosette Nanotubes: Hydrogen Bond Network and Channel-Bound Water
HighField Solid-State NMR Spectroscopy Investigation of 15N-Labeled Rosette Nanotubes: Hydrogen Bond Network and Channel-Bound Water
Hicham Fenniri, Grigory A. Tikhomirov, Darren H. Brouwer, Souhaila Bouatra, Mounir El Bakkari, Zhimin Yan, Jae-Young Cho and Takeshi Yamazaki
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.6b02420/20160505/images/medium/ja-2016-02420c_0005.gif
Journal of the American Chemical Society
DOI: 10.1021/jacs.6b02420
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA...
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(15)N and (1)H Solid-State NMR Investigation of a Canonical Low-Barrier Hydrogen-Bond Compound: 1,8-bis(dimethylamino) Naphthalene.
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J Phys Chem B. 2015 Aug 5;
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Abstract
Strong or low-barrier hydrogen bonds have been often proposed in proteins to explain enzyme catalysis and proton transfer reactions. So far 1H chemical shifts and scalar couplings have been used as the main...
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http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.rsc.org-images-entities-char_z_RSClogo.gif Related Articles Determining hydrogen-bond interactions in spider silk with 1H-13C HETCOR fast MAS solid-state NMR and DFT proton chemical shift calculations.
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Authors: Holland GP, Mou Q, Yarger JL
Abstract
Two-dimensional (2D) (1)H-(13)C...
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Chemphyschem. 2013 Apr 15;
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Abstract
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Abstract Solid-state NMR has emerged as an important tool for structural biology and chemistry, capable of solving atomic-resolution structures for proteins in membrane-bound and aggregated states. Proton detection methods have been recently realized under fast magic-angle spinning conditions, providing large sensitivity enhancements for efficient examination of uniformly labeled proteins. The first and often most challenging step of protein structure determination by NMR is the...
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Proton-Detected Solid-State NMR Spectroscopy of Fibrillar and Membrane Proteins.
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Authors: Kuwata K, Matumoto T, Cheng H, Nagayama K, James TL, Roder H
PrP106-126, a peptide corresponding to residues 107-127 of the human prion protein, induces neuronal cell...
Hydrogen bond strength in membrane proteins by time-resolved 1H-detected solid-state NMR and MD simulations
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