High-throughput screening of structural proteomics targets using NMR.
 

High-throughput screening of structural proteomics targets using NMR.

Related Articles High-throughput screening of structural proteomics targets using NMR.

FEBS Lett. 2003 Sep 25;552(2-3):207-13

Authors: Galvão-Botton LM, Katsuyama AM, Guzzo CR, Almeida FC, Farah CS, Valente AP

We applied a high-throughput strategy for the screening of targets for structural proteomics of Xanthomonas axonopodis pv citri. This strategy is based on the rapid (1)H-(15)N HSQC NMR analysis of bacterial lysates containing selectively (15)N-labelled heterologous proteins. Our analysis permitted us to classify the 19 soluble candidates in terms of 'foldedness', that is, the extent to which they present a well-folded solution structure, as reflected by the quality of their NMR spectra. This classification allowed us to define a priority list to be used as a guide to select protein candidates for further structural studies.

PMID: 14527688 [PubMed - indexed for MEDLINE]



Source: PubMed