Related ArticlesHigh-Resolution NMR of Folded Proteins in Hyperpolarized Physiological Solvents.
Chemistry. 2018 Jul 03;:
Authors: Kaderavek P, Ferrage F, Bodenhausen G, Kurzbach D
Abstract
We report hyperpolarized 2D exchange spectroscopy (HYPEX) to obtain high-resolution nuclear magnetic resonance (NMR) spectra of folded proteins under near-physiological conditions. The technique is based on hyperpolarized water, which is prepared by dissolution dynamic nuclear polarization and mixed in-situ in an NMR spectrometer with a protein in a physiological saline buffer at body temperature. We show that rapid exchange of labile protons with the hyperpolarized solvent, combined with cross-relaxation effects (NOEs), leads to boosted signal intensities for many amide 1H-15N resonances in the protein ubiquitin. As the introduction of hyperpolarization to the target protein is mediated via the solvent, the method is applicable to a broad spectrum of target molecules.
PMID: 29969165 [PubMed - as supplied by publisher]
[NMR paper] High-resolution 2D NMR of disordered proteins enhanced by hyperpolarized water.
High-resolution 2D NMR of disordered proteins enhanced by hyperpolarized water.
High-resolution 2D NMR of disordered proteins enhanced by hyperpolarized water.
Anal Chem. 2018 Mar 12;:
Authors: Szekely O, Olsen GL, Felli IC, Frydman L
Abstract
This study demonstrates the usefulness derived from relying on hyperpolarized water obtained by dissolution DNP, for site-resolved biophysical NMR studies of intrinsically disordered proteins. Thanks to the facile amide-solvent exchange experienced by protons in these proteins, 2D NMR...
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03-13-2018 07:39 PM
High-resolution hyperpolarized metabolic imaging of the rat heart using k-t PCA and k-t SPARSE
From The DNP-NMR Blog:
High-resolution hyperpolarized metabolic imaging of the rat heart using k-t PCA and k-t SPARSE
Wespi, P., et al., High-resolution hyperpolarized metabolic imaging of the rat heart using k-t PCA and k-t SPARSE. NMR Biomed., 2018. 31(2): p. e3876-n/a.
https://www.ncbi.nlm.nih.gov/pubmed/29244228
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03-07-2018 08:27 PM
High-resolution hyperpolarized metabolic imaging of the rat heart using k-t PCA and k-t SPARSE
From The DNP-NMR Blog:
High-resolution hyperpolarized metabolic imaging of the rat heart using k-t PCA and k-t SPARSE
Wespi, P., et al., High-resolution hyperpolarized metabolic imaging of the rat heart using k-t PCA and k-t SPARSE. NMR Biomed, 2018. 31(2): p. e3876-n/a.
https://www.ncbi.nlm.nih.gov/pubmed/29244228
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02-26-2018 04:18 PM
High-resolution hyperpolarized in vivo metabolic 13C spectroscopy at low magnetic field (48.7mT) following murine tail-vein injection
From The DNP-NMR Blog:
High-resolution hyperpolarized in vivo metabolic 13C spectroscopy at low magnetic field (48.7mT) following murine tail-vein injection
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Coffey, A.M., et al., High-resolution hyperpolarized in vivo metabolic 13C spectroscopy at low magnetic field (48.7mT) following murine tail-vein injection. J. Magn. Reson., 2017. 281(Supplement C): p. 246-252.
http://www.sciencedirect.com/science/article/pii/S1090780717301659
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11-18-2017 07:08 AM
High-resolution 3D proton MRI of hyperpolarized gas enabled by parahydrogen and Rh/TiO2 heterogeneous catalyst
From The DNP-NMR Blog:
High-resolution 3D proton MRI of hyperpolarized gas enabled by parahydrogen and Rh/TiO2 heterogeneous catalyst
Kovtunov, K.V., et al., High-resolution 3D proton MRI of hyperpolarized gas enabled by parahydrogen and Rh/TiO2 heterogeneous catalyst. Chemistry, 2014. 20(37): p. 11636-9.
http://www.ncbi.nlm.nih.gov/pubmed/24961814
High dimensional and high resolution pulse sequences for backbone resonance assignment of intrinsically disordered proteins
High dimensional and high resolution pulse sequences for backbone resonance assignment of intrinsically disordered proteins
Abstract Four novel 5D (HACA(N)CONH, HNCOCACB, (HACA)CON(CA)CONH, (H)NCO(NCA)CONH), and one 6D ((H)NCO(N)CACONH) NMR pulse sequences are proposed. The new experiments employ non-uniform sampling that enables achieving high resolution in indirectly detected dimensions. The experiments facilitate resonance assignment of intrinsically disordered proteins. The novel pulse sequences were successfully tested using δ subunit (20 kDa) of Bacillus subtilis RNA polymerase...
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02-21-2012 03:40 AM
High resolution NMR spectroscopy of nanocrystalline proteins at ultra-high magnetic field
High resolution NMR spectroscopy of nanocrystalline proteins at ultra-high magnetic field
Abstract Magic-angle spinning (MAS) solid-state NMR (SSNMR) spectroscopy of uniformly-13C,15N labeled protein samples provides insight into atomic-resolution chemistry and structure. Data collection efficiency has advanced remarkably in the last decade; however, the study of larger proteins is still challenged by relatively low resolution in comparison to solution NMR. In this study, we present a systematic analysis of SSNMR protein spectra acquired at 11.7, 17.6 and 21.1 Tesla (1H frequencies of...
High-Resolution NMR of Folded Proteins in Hyperpolarized Physiological Solvents.
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