High-Resolution NMR Determination of the Dynamic Structure of Membrane Proteins.
 

High-Resolution NMR Determination of the Dynamic Structure of Membrane Proteins.

High-Resolution NMR Determination of the Dynamic Structure of Membrane Proteins.

Angew Chem Int Ed Engl. 2016 Jul 27;

Authors: Jaremko M, Jaremko ?, Villinger S, Schmidt CD, Griesinger C, Becker S, Zweckstetter M

Abstract
(15) N spin-relaxation rates are demonstrated to provide critical information about the long-range structure and internal motions of membrane proteins. Combined with an improved calculation method, the relaxation-rate-derived structure of the 283-residue human voltage-dependent anion channel revealed an anisotropically shaped barrel with a rigidly attached N-terminal helix. Our study thus establishes an NMR spectroscopic approach to determine the structure and dynamics of mammalian membrane proteins at high accuracy and resolution.


PMID: 27461260 [PubMed - as supplied by publisher]



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