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Default High-resolution 31p field cycling NMR as a probe of phospholipid dynamics.

High-resolution 31p field cycling NMR as a probe of phospholipid dynamics.

Related Articles High-resolution 31p field cycling NMR as a probe of phospholipid dynamics.

J Am Chem Soc. 2004 Oct 27;126(42):13765-77

Authors: Roberts MF, Redfield AG

We have used high-resolution field-cycling 31P NMR spectroscopy to measure spin-lattice relaxation rates (R1 = 1/T1) of multicomponent phospholipid vesicle and micelle samples over a large field range, from 0.1 to 11.7 T. The shape of the curve for R1 as a function of field and a model-free analysis were used to extract tauc, a correlation time for each type of phospholipid molecule in the bilayer that is likely to reflect rotation of the molecule about the axis perpendicular to the membrane surface; Sc2, a chemical shift anisotropy (CSA) order parameter; and tauhf, a time constant reflecting faster internal motion. This 31P technique was also used to monitor association of a peripheral membrane protein, Bacillus thuringiensis phosphatidylinositol-specific phospholipase C, with both phosphatidylcholine and phosphatidylmethanol bilayers. Differences in phospholipid dynamics induced by the protein shed light on how zwitterionic phosphatidylcholine, and not the anionic phosphatidylmethanol, activates the enzyme toward its substrate.

PMID: 15493936 [PubMed - indexed for MEDLINE]



Source: PubMed
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