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nmrlearner 03-30-2017 06:42 PM

High-pressure NMR techniques for the study of protein dynamics, folding and aggregation
 
High-pressure NMR techniques for the study of protein dynamics, folding and aggregation

Publication date: April 2017
Source:Journal of Magnetic Resonance, Volume 277</br>
Author(s): Luan M. Nguyen, Julien Roche</br>
High-pressure is a well-known perturbation method used to destabilize globular proteins and dissociate protein complexes or aggregates. The heterogeneity of the response to pressure offers a unique opportunity to dissect the thermodynamic contributions to protein stability. In addition, pressure perturbation is generally reversible, which is essential for a proper thermodynamic characterization of a protein equilibrium. When combined with NMR spectroscopy, hydrostatic pressure offers the possibility of monitoring at an atomic resolution the structural transitions occurring upon unfolding and determining the kinetic properties of the process. The recent development of commercially available high-pressure sample cells greatly increased the potential applications for high-pressure NMR experiments that can now be routinely performed. This review summarizes the recent applications and future directions of high-pressure NMR techniques for the characterization of protein conformational fluctuations, protein folding and the stability of protein complexes and aggregates.
Graphical abstract

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