High dimensional and high resolution pulse sequences for backbone resonance assignment of intrinsically disordered proteins
 

High dimensional and high resolution pulse sequences for backbone resonance assignment of intrinsically disordered proteins


Abstract Four novel 5D (HACA(N)CONH, HNCOCACB, (HACA)CON(CA)CONH, (H)NCO(NCA)CONH), and one 6D ((H)NCO(N)CACONH) NMR pulse sequences are proposed. The new experiments employ non-uniform sampling that enables achieving high resolution in indirectly detected dimensions. The experiments facilitate resonance assignment of intrinsically disordered proteins. The novel pulse sequences were successfully tested using δ subunit (20 kDa) of Bacillus subtilis RNA polymerase that has an 81-amino acid disordered part containing various repetitive sequences.

• Content Type Journal Article
• Category Article
• Pages 1-9
• DOI 10.1007/s10858-012-9613-x
• Authors
  • Anna Zawadzka-Kazimierczuk, Faculty of Chemistry, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland
  • Wiktor KoźmiÅ?ski, Faculty of Chemistry, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland
  • Hana Å*anderová, Laboratory of Molecular Genetics of Bacteria, Department of Bacteriology, Institute of Microbiology, Academy of Sciences of the Czech Republic, VÃ*deÅ?ská 1083, 142 20 Prague, Czech Republic
  • Libor Krásný, Laboratory of Molecular Genetics of Bacteria, Department of Bacteriology, Institute of Microbiology, Academy of Sciences of the Czech Republic, VÃ*deÅ?ská 1083, 142 20 Prague, Czech Republic

• • Journal Journal of Biomolecular NMR
  • Online ISSN 1573-5001
  • Print ISSN 0925-2738

Source: Journal of Biomolecular NMR