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NMR processing:
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Side-chains:
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NOEs:
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UNIO Candid
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Ab initio:
GeNMR
Cyana
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ASDP
UNIO ATNOS-Candid
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Fragment-based:
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Template-based:
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Refinement:
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Structure from chemical shifts:
Fragment-based:
WeNMR CS-Rosetta
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Homology-based:
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Torsion angles from chemical shifts:
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Secondary structure from chemical shifts:
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Flexibility from chemical shifts:
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Methyl S2
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Molecular dynamics:
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From structure:
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ArShift- Aromatic
ShiftS
Proshift
PPM
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From sequence:
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Poulsen_rc_CS
Disordered proteins:
MAXOCC
Format conversion & validation:
CCPN
From NMR-STAR 3.1
Validate NMR-STAR 3.1
NMR sample preparation:
Protein disorder:
DisMeta
Protein solubility:
camLILA
ccSOL
Camfold
camGroEL
Zyggregator
Isotope labeling:
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Solid-state NMR:
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Default Heteronuclear solution-state NMR studies of the chromophore in cyanobacterial phytoch

Heteronuclear solution-state NMR studies of the chromophore in cyanobacterial phytochrome Cph1.

Related Articles Heteronuclear solution-state NMR studies of the chromophore in cyanobacterial phytochrome Cph1.

Biochemistry. 2005 Jun 14;44(23):8244-50

Authors: Strauss HM, Hughes J, Schmieder P

Precise structural information regarding the chromophore binding pocket is essential for an understanding of photochromicity and photoconversion in phytochrome photoreceptors. To this end, we are studying the 59 kDa N-terminal module of the cyanobacterial phytochrome Cph1 from Synechocystis sp. PCC 6803 in both thermally stable forms (Pr and Pfr) using solution-state NMR spectroscopy. The protein is deuterated, while the chromophore, phycocyanobilin (PCB), is isotopically labeled with (15)N or (13)C and (15)N. We have established a simple approach for preparing labeled PCB based on BG11 medium supplemented with an appropriate buffer and NaH(13)CO(3) and Na(15)NO(3) as sole carbon and nitrogen sources, respectively. We show that structural details of the chromophore binding pocket in both Pr and Pfr forms can be obtained using multidimensional heteronuclear solution-state NMR spectroscopy. Using one-dimensional (15)N NMR spectra, we show unequivocally that the chromophore is protonated in both Pr and Pfr states.

PMID: 15938613 [PubMed - indexed for MEDLINE]



Source: PubMed
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