[NMR paper] Facilitated Assignment of Large Protein NMR Signals with Covariance Sequential Spectra Using Spectral Derivatives.
Facilitated Assignment of Large Protein NMR Signals with Covariance Sequential Spectra Using Spectral Derivatives.
Facilitated Assignment of Large Protein NMR Signals with Covariance Sequential Spectra Using Spectral Derivatives.
J Am Chem Soc. 2014 Sep 16;
Authors: Harden BJ, Nichols SR, Frueh DP
Abstract
Nuclear magnetic resonance (NMR) studies of larger proteins are hampered by difficulties in assigning NMR resonances. Human intervention is typically required to identify NMR signals in 3D spectra, and subsequent procedures...
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09-17-2014 11:54 AM
Solid state NMR chemical shift assignment and conformational analysis of a cellulose binding protein facilitated by optimized glycerol enrichment
Solid state NMR chemical shift assignment and conformational analysis of a cellulose binding protein facilitated by optimized glycerol enrichment
Abstract
Magic-angle spinning solid-state NMR has been applied to study CBM3bâ??Cbh9A (CBM3b), a cellulose binding module protein belonging to family 3b. It is a 146-residue protein having a unique nine-stranded β-sandwich fold, in which 35Â*% of the structure is in a β-sheet conformation and the remainder of the protein is composed of loops and unstructured regions. Yet, the protein can be crystalized...
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06-19-2014 10:21 PM
[NMR paper] Solid state NMR chemical shift assignment and conformational analysis of a cellulose binding protein facilitated by optimized glycerol enrichment.
Solid state NMR chemical shift assignment and conformational analysis of a cellulose binding protein facilitated by optimized glycerol enrichment.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles Solid state NMR chemical shift assignment and conformational analysis of a cellulose binding protein facilitated by optimized glycerol enrichment.
J Biomol NMR. 2014 May 14;
Authors: Ivanir H, Goldbourt A
Abstract
Magic-angle spinning...
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05-16-2014 08:06 PM
[NMR paper] Two-dimensional NMR spectroscopy reveals cation-triggered backbone degradation in polysulfone-based anion exchange membranes.
Two-dimensional NMR spectroscopy reveals cation-triggered backbone degradation in polysulfone-based anion exchange membranes.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--highwire.stanford.edu-icons-externalservices-pubmed-custom-pnas_full.gif Related Articles Two-dimensional NMR spectroscopy reveals cation-triggered backbone degradation in polysulfone-based anion exchange membranes.
Proc Natl Acad Sci U S A. 2013 Feb 12;110(7):2490-5
Authors: Arges CG, Ramani V
Abstract
Anion exchange membranes (AEMs) find...
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04-09-2013 06:31 PM
[Question from NMRWiki Q&A forum] How to study polymer degradation by gamma and X-Ray radiation?
How to study polymer degradation by gamma and X-Ray radiation?
Hi, I was asked to study polyethylene degradated by gamma and Xrays. I planed to measure relaxations rates, but which one is the most relevant to highlight chain degradation? T1, T2, but on 1H or 13C? By reading some articles, I feel T2 is not very interesting, right? T1rho experiments seems to give good results, I have found 2 sequences in Bruker's library, T1rhoX (CP step then variable length spin lock pulse on 13C channel) and T1rhoH (CP step then variable length spin lock pulse on 1H channel), and there is also the more...
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03-16-2011 06:24 PM
[Question from NMRWiki Q&A forum] study of polymer degradation
study of polymer degradation
Hi, I was asked to study polyethylene degradated by gamma and Xrays. I planed to measure relaxations rates, but which one is the most relevant to highlight chain degradation? T1, T2, but on 1H or 13C? By reading some articles, I feel T2 is not very interesting, right? T1rho experiments seems to give good results, I have found 2 sequences in Bruker's library, T1rhoX (CP step then variable length spin lock pulse on 13C channel) and T1rhoH (CP step then variable length spin lock pulse on 1H channel), and there is also the more classical variable contact time...
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03-16-2011 06:14 AM
[Question from NMRWiki Q&A forum] Examples of protein degradation eg HSQC?
Examples of protein degradation eg HSQC?
Anyone know any websites or papers that show protein degradation in an HSQC for example? I wanted to know what to look for in old samples when I have them. Either that, or could someone put one up on the wiki for me to look at?
Check if somebody has answered this question on NMRWiki QA forum
The ssrA-Tag Facilitated Degradation of an IntegralMembrane Protein
Linear Mode
