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Default Haemolymph from Mytilus galloprovincialis: Response to copper and temperature challenges studied by 1H-NMR metabonomics.

Haemolymph from Mytilus galloprovincialis: Response to copper and temperature challenges studied by 1H-NMR metabonomics.

Related Articles Haemolymph from Mytilus galloprovincialis: Response to copper and temperature challenges studied by 1H-NMR metabonomics.

Comp Biochem Physiol C Toxicol Pharmacol. 2016 Feb 17;

Authors: Digilio G, Sforzini S, Cassino C, Robotti E, Oliveri C, Marengo E, Musso D, Osella D, Viarengo A

Abstract
Numerous studies on molluscs have been carried out to clarify the physiological roles of haemolymph serum proteins and haemocytes. However, little is known about the presence and functional role of the serum metabolites. In this study, Nuclear Magnetic Resonance (NMR) was used to assess whether changes of the metabolic profile of Mytilus galloprovincialis haemolymph may reflect alterations of the physiological status of the organisms due to environmental stressors, namely copper and temperature. Mussel haemolymph was taken from the posterior adductor muscle after a 4-day exposure to ambient (16°C) or high temperature (24°C) and in the absence or presence (5?g/L, 20?g/L, or 40?g/L) of sublethal copper (Cu(2+)). The total glutathione (GSH) concentration in the haemolymph of both control and treated mussels was minimal, indicating the absence of significant contaminations by muscle intracellular metabolites due to the sampling procedure. In the 1H-NMR spectrum of haemolymph, 27 metabolites were identified unambiguously. The separate and combined effects of exposure to copper and temperature on the haemolymph metabolic profile were assessed by Principal Component Analysis (PCA) and Ranking-PCA multivariate analysis. Changes of the metabolomic profile due to copper exposure at 16°C became detectable at a dose of 20?g/L copper. Alanine, lysine, serine, glutamine, glycogen, glucose and protein aliphatics played a major role in the classification of the metabolic changes according to the level of copper exposition. High temperature (24°C) and high copper levels caused a coherent increase of a common set of metabolites (mostly glucose, serine, and lysine), indicating that the metabolic impairment due to high temperature is enforced by the presence of copper. Overall, the results demonstrate that, as for human blood plasma, the analysis of haemolymph metabolites represents a promising tool for the diagnosis of pollutant-induced stress syndrome in marine mussels.


PMID: 26899427 [PubMed - as supplied by publisher]



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