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-   -   [NMR paper] Gradient reconstitution of membrane proteins for solid-state NMR studies. (http://www.bionmr.com/forum/journal-club-9/gradient-reconstitution-membrane-proteins-solid-state-nmr-studies-25058/)

nmrlearner 09-14-2017 11:59 AM

Gradient reconstitution of membrane proteins for solid-state NMR studies.
 
Gradient reconstitution of membrane proteins for solid-state NMR studies.

Related Articles Gradient reconstitution of membrane proteins for solid-state NMR studies.

J Biomol NMR. 2017 Sep 12;:

Authors: Lacabanne D, Lends A, Danis C, Kunert B, Fogeron ML, Jirasko V, Chuilon C, Lecoq L, Orelle C, Chaptal V, Falson P, Jault JM, Meier BH, Böckmann A

Abstract
We here adapted the GRecon method used in electron microscopy studies for membrane protein reconstitution to the needs of solid-state NMR sample preparation. We followed in detail the reconstitution of the ABC transporter BmrA by dialysis as a reference, and established optimal reconstitution conditions using the combined sucrose/cyclodextrin/lipid gradient characterizing GRecon. We established conditions under which quantitative reconstitution of active protein at low lipid-to-protein ratios can be obtained, and also how to upscale these conditions in order to produce adequate amounts for NMR. NMR spectra recorded on a sample produced by GRecon showed a highly similar fingerprint as those recorded previously on samples reconstituted by dialysis. GRecon sample preparation presents a gain in time of nearly an order of magnitude for reconstitution, and shall represent a valuable alternative in solid-state NMR membrane protein sample preparation.


PMID: 28900789 [PubMed - as supplied by publisher]



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