Genotype evaluation of cowpea seeds (Vigna unguiculata) using (1)H qNMR combined with exploratory tools and solid-state NMR.
 

Genotype evaluation of cowpea seeds (Vigna unguiculata) using (1)H qNMR combined with exploratory tools and solid-state NMR.

Related Articles Genotype evaluation of cowpea seeds (Vigna unguiculata) using (1)H qNMR combined with exploratory tools and solid-state NMR.

Food Res Int. 2017 Jan;91:140-147

Authors: Alves Filho EG, Silva LM, Teofilo EM, Larsen FH, de Brito ES

Abstract
The ultimate aim of this study was to apply a non-targeted chemometric analysis (principal component analysis and hierarchical clustering analysis using the heat map approach) of NMR data to investigate the variability of organic compounds in nine genotype cowpea seeds, without any complex pre-treatment. In general, both exploratory tools show that Tvu 233, CE-584, and Setentão genotypes presented higher amount mainly of raffinose and Tvu 382 presented the highest content of choline and least content of raffinose. The evaluation of the aromatic region showed the Setentão genotype with highest content of niacin/vitamin B3 whereas Tvu 382 with lowest amount. To investigate rigid and mobile components in the seeds cotyledon, (13)C CP and SP/MAS solid-state NMR experiments were performed. The cotyledon of the cowpea comprised a rigid part consisting of starch as well as a soft portion made of starch, fatty acids, and protein. The variable contact time experiment suggests the presence of lipid-amylose complexes.


PMID: 28290318 [PubMed - in process]



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