Genetically encoded site-specific (19)F unnatural amino acid incorporation in V. natriegens for in-cell NMR analysis
 

Genetically encoded site-specific (19)F unnatural amino acid incorporation in V. natriegens for in-cell NMR analysis

Nuclear magnetic resonance (NMR) spectroscopy NMR is a well-established technique for probing protein structure, dynamics and conformational changes. Taking advantage of the high signal sensitivity and broad chemical shift range of ^(19)F nuclei, ^(19)F NMR has been applied to investigate protein function at atomic resolution. In this report, we extend the unnatural amino acid site-specific incorporation into V. natriegens, an alternate protein expression system. The unnatural amino acid...

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