Related ArticlesFunctional Analysis of the Nitrogen Metabolite Repression Regulator Gene nmrA in Aspergillus flavus.
Front Microbiol. 2016;7:1794
Authors: Han X, Qiu M, Wang B, Yin WB, Nie X, Qin Q, Ren S, Yang K, Zhang F, Zhuang Z, Wang S
Abstract
In Aspergillus nidulans, the nitrogen metabolite repression (NMR) regulator NmrA plays a major role in regulating the activity of the GATA transcription factor AreA during nitrogen metabolism. However, the function of nmrA in A. flavus has not been previously studied. Here, we report the identification and functional analysis of nmrA in A. flavus. Our work showed that the amino acid sequences of NmrA are highly conserved among Aspergillus species and that A. flavus NmrA protein contains a canonical Rossmann fold motif. Deletion of nmrA slowed the growth of A. flavus but significantly increased conidiation and sclerotia production. Moreover, seed infection experiments indicated that nmrA is required for the invasive virulence of A. flavus. In addition, the ?nmrA mutant showed increased sensitivity to rapamycin and methyl methanesulfonate, suggesting that nmrA could be responsive to target of rapamycin signaling and DNA damage. Furthermore, quantitative real-time reverse transcription polymerase chain reaction analysis suggested that nmrA might interact with other nitrogen regulatory and catabolic genes. Our study provides a better understanding of NMR and the nitrogen metabolism network in fungi.
[NMR paper] Solution NMR structure of CsgE: Structural insights into a chaperone and regulator protein important for functional amyloid formation.
Solution NMR structure of CsgE: Structural insights into a chaperone and regulator protein important for functional amyloid formation.
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Proc Natl Acad Sci U S A. 2016 Jun 13;
Authors: Shu Q, Krezel AM, Cusumano ZT, Pinkner JS, Klein R, Hultgren SJ, Frieden C
Abstract
Curli, consisting primarily of major structural subunit CsgA, are functional amyloids produced on the surface of...
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06-15-2016 11:12 PM
[NMR paper] Systematic identification of protein-metabolite interactions in complex metabolite mixtures by ligand-detected NMR spectroscopy.
Systematic identification of protein-metabolite interactions in complex metabolite mixtures by ligand-detected NMR spectroscopy.
Systematic identification of protein-metabolite interactions in complex metabolite mixtures by ligand-detected NMR spectroscopy.
Biochemistry. 2016 Apr 11;
Authors: Nikolaev YV, Kochanowski K, Link H, Sauer U, Allain FH
Abstract
Protein-metabolite interactions play a vital role in the regulation of numerous cellular processes. Consequently, identifying such interactions is a key prerequisite for...
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[NMR paper] Correlating high-resolution magic angle spinning NMR spectroscopy and gene analysis in osteoarthritic cartilage.
Correlating high-resolution magic angle spinning NMR spectroscopy and gene analysis in osteoarthritic cartilage.
Correlating high-resolution magic angle spinning NMR spectroscopy and gene analysis in osteoarthritic cartilage.
NMR Biomed. 2015 Mar 12;
Authors: Tufts L, Shet Vishnudas K, Fu E, Kurhanewicz J, Ries M, Alliston T, Li X
Abstract
Osteoarthritis (OA) is a common multifactorial and heterogeneous degenerative joint disease, and biochemical changes in cartilage matrix occur during the early stages of OA before...
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03-13-2015 12:31 PM
[NMR paper] Solution NMR Structure and Functional Analysis of the Integral Membrane Protein YgaP from E. coli.
Solution NMR Structure and Functional Analysis of the Integral Membrane Protein YgaP from E. coli.
Solution NMR Structure and Functional Analysis of the Integral Membrane Protein YgaP from E. coli.
J Biol Chem. 2014 Jun 23;
Authors: Eichmann C, Tzitzilonis C, Bordignon E, Maslennikov I, Choe S, Riek R
Abstract
The solution NMR structure of the ?-helical integral membrane protein YgaP from Escherichia coli in mixed DHPC-7/LMPG micelles is presented. In these micelles, YgaP forms a homo-dimer with the two transmembrane helices...
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[NMR paper] Nitrogen-15 NMR studies of nitrogen metabolism in Picea glauca buds.
Nitrogen-15 NMR studies of nitrogen metabolism in Picea glauca buds.
Related Articles Nitrogen-15 NMR studies of nitrogen metabolism in Picea glauca buds.
Plant Physiol Biochem. 2004 Dec;42(10):803-9
Authors: Bagh K, Hiraoki T, Thorpe TA, Vogel HJ
In vivo (15)N nuclear magnetic resonance (NMR) as well as (15)N solid-state magic angle spinning (MAS) NMR spectroscopy were used to investigate nitrogen metabolism in cultured white spruce (Picea glauca) buds. Long-term as well as short-term experiments were carried out involving the use of...
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11-24-2010 10:03 PM
[NMR paper] Molecular comparison of the negative-acting nitrogen control gene, nmr, in Neurospora
Molecular comparison of the negative-acting nitrogen control gene, nmr, in Neurospora crassa and other Neurospora and fungal species.
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Biochem Genet. 1991 Oct;29(9-10):447-59
Authors: Young JL, Marzluf GA
In Neurospora crassa, the expression of unlinked structural genes which encode nitrogen catabolic enzymes is subject to genetic and metabolic regulation. The negative-acting nmr regulatory gene...
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[NMR paper] Molecular comparison of the negative-acting nitrogen control gene, nmr, in Neurospora
Molecular comparison of the negative-acting nitrogen control gene, nmr, in Neurospora crassa and other Neurospora and fungal species.
Related Articles Molecular comparison of the negative-acting nitrogen control gene, nmr, in Neurospora crassa and other Neurospora and fungal species.
Biochem Genet. 1991 Oct;29(9-10):447-59
Authors: Young JL, Marzluf GA
In Neurospora crassa, the expression of unlinked structural genes which encode nitrogen catabolic enzymes is subject to genetic and metabolic regulation. The negative-acting nmr regulatory gene...
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[NMR paper] Nucleotide sequence and analysis of NMR, a negative-acting regulatory gene in the nit
Nucleotide sequence and analysis of NMR, a negative-acting regulatory gene in the nitrogen circuit of Neurospora crassa.
Related Articles Nucleotide sequence and analysis of NMR, a negative-acting regulatory gene in the nitrogen circuit of Neurospora crassa.
Mol Gen Genet. 1990 Jun;222(1):120-8
Authors: Young JL, Jarai G, Fu YH, Marzluf GA
In Neurospora the expression of a set of unlinked structural genes, which allows utilization of various nitrogen-containing compounds, is controlled by the positive-acting nit-2 gene and the negative-acting...
Functional Analysis of the Nitrogen Metabolite Repression Regulator Gene nmrA in Aspergillus flavus.
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