Related ArticlesFormation kinetics and structural features of Beta-amyloid aggregates by sedimented solute NMR.
Chembiochem. 2013 Sep 23;14(14):1891-7
Authors: Bertini I, Gallo G, Korsak M, Luchinat C, Mao J, Ravera E
Abstract
The accumulation of soluble toxic beta-amyloid (A?) aggregates is an attractive hypothesis for the role of this peptide in the pathology of Alzheimer's disease. We have introduced sedimentation through ultracentrifugation, either by magic angle spinning (in situ) or preparative ultracentrifuge (ex situ), to immobilize biomolecules and make them amenable for solid-state NMR studies (SedNMR). In situ SedNMR is used here to address the kinetics of formation of soluble A? assemblies by monitoring the disappearance of the monomer and the appearance of the oligomers simultaneously. Ex situ SedNMR allows us to select different oligomeric species and to reveal atomic-level structural features of soluble A? assemblies.
The Feasibilityof Formation and Kinetics of NMR SignalAmplification by Reversible Exchange (SABRE) at High Magnetic Field(9.4 T)
The Feasibilityof Formation and Kinetics of NMR SignalAmplification by Reversible Exchange (SABRE) at High Magnetic Field(9.4 T)
Danila A. Barskiy, Kirill V. Kovtunov, Igor V. Koptyug, Ping He, Kirsten A. Groome, Quinn A. Best, Fan Shi, Boyd M. Goodson, Roman V. Shchepin, Aaron M. Coffey, Kevin W. Waddell and Eduard Y. Chekmenev
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja501052p/aop/images/medium/ja-2014-01052p_0005.gif
Journal of the American Chemical Society
DOI: 10.1021/ja501052p...
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[NMR paper] On the use of ultracentrifugal devices for sedimented solute NMR.
On the use of ultracentrifugal devices for sedimented solute NMR.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles On the use of ultracentrifugal devices for sedimented solute NMR.
J Biomol NMR. 2012 Oct;54(2):123-7
Authors: Bertini I, Engelke F, Gonnelli L, Knott B, Luchinat C, Osen D, Ravera E
Abstract
We have recently proposed sedimented solute NMR (SedNMR) as a solid-state method to access biomolecules without the need of crystallization or other...
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02-13-2013 12:47 PM
On the use of ultracentrifugal devices for sedimented solute NMR
On the use of ultracentrifugal devices for sedimented solute NMR
Abstract We have recently proposed sedimented solute NMR (SedNMR) as a solid-state method to access biomolecules without the need of crystallization or other sample manipulation. The drawback of SedNMR is that samples are intrinsically diluted and this is detrimental for the signal intensity. Ultracentrifugal devices can be used to increase the amount of sample inside the rotor, overcoming the intrinsic sensitivity limitation of the method. We designed two different devices and we here report the directions for using...
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Covalent structural changes in unfolded GroES that lead to amyloid fibril formation detected by NMR: Insight into intrinsically disordered proteins.
Covalent structural changes in unfolded GroES that lead to amyloid fibril formation detected by NMR: Insight into intrinsically disordered proteins.
Covalent structural changes in unfolded GroES that lead to amyloid fibril formation detected by NMR: Insight into intrinsically disordered proteins.
J Biol Chem. 2011 Apr 20;
Authors: Iwasa H, Meshitsuka S, Hongo K, Mizobata T, Kawata Y
Co-chaperonin GroES from E. coli works with chaperonin GroEL to mediate the folding reactions of various proteins. However, under specific conditions, i. e., the...
[NMR paper] Amyloid fibril formation by A beta 16-22, a seven-residue fragment of the Alzheimer's
Amyloid fibril formation by A beta 16-22, a seven-residue fragment of the Alzheimer's beta-amyloid peptide, and structural characterization by solid state NMR.
Related Articles Amyloid fibril formation by A beta 16-22, a seven-residue fragment of the Alzheimer's beta-amyloid peptide, and structural characterization by solid state NMR.
Biochemistry. 2000 Nov 14;39(45):13748-59
Authors: Balbach JJ, Ishii Y, Antzutkin ON, Leapman RD, Rizzo NW, Dyda F, Reed J, Tycko R
The seven-residue peptide N-acetyl-Lys-Leu-Val-Phe-Phe-Ala-Glu-NH(2), called A...
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[NMR paper] An unusual peptide conformation may precipitate amyloid formation in Alzheimer's dise
An unusual peptide conformation may precipitate amyloid formation in Alzheimer's disease: application of solid-state NMR to the determination of protein secondary structure.
Related Articles An unusual peptide conformation may precipitate amyloid formation in Alzheimer's disease: application of solid-state NMR to the determination of protein secondary structure.
Biochemistry. 1991 Oct 29;30(43):10382-7
Authors: Spencer RG, Halverson KJ, Auger M, McDermott AE, Griffin RG, Lansbury PT
The formation of insoluble proteinaceous deposits is...
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[NMR paper] An unusual peptide conformation may precipitate amyloid formation in Alzheimer's dise
An unusual peptide conformation may precipitate amyloid formation in Alzheimer's disease: application of solid-state NMR to the determination of protein secondary structure.
Related Articles An unusual peptide conformation may precipitate amyloid formation in Alzheimer's disease: application of solid-state NMR to the determination of protein secondary structure.
Biochemistry. 1991 Oct 29;30(43):10382-7
Authors: Spencer RG, Halverson KJ, Auger M, McDermott AE, Griffin RG, Lansbury PT
The formation of insoluble proteinaceous deposits is...
Formation kinetics and structural features of Beta-amyloid aggregates by sedimented solute NMR.
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