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Default Folding of a beta-sheet protein monitored by real-time NMR spectroscopy.

Folding of a beta-sheet protein monitored by real-time NMR spectroscopy.

Related Articles Folding of a beta-sheet protein monitored by real-time NMR spectroscopy.

J Mol Biol. 2003 May 16;328(5):1161-71

Authors: Mizuguchi M, Kroon GJ, Wright PE, Dyson HJ

At low ionic strength, apoplastocyanin forms an unfolded state under non-denaturing conditions. The refolding of this state is sufficiently slow to allow real-time NMR experiments to be performed. Folding of apoplastocyanin, initiated by the addition of salt and followed by real-time 2D 1H-15N heteronuclear single quantum coherence (HSQC) spectroscopy, is highly cooperative. A concomitant increase in the intensity of both sequential and long-range nuclear Overhauser effects (NOEs) between backbone amide protons in successive acquisitions of 1H-15N HSQC-NOESY-HSQC spectra provides the first direct observation of the development of structure-specific NOEs as a protein folds. Our results show that the local and long-range interactions in the native apoplastocyanin are formed simultaneously, consistent with highly cooperative formation of the native structure.

PMID: 12729749 [PubMed - indexed for MEDLINE]



Source: PubMed
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