The determination of the binding affinity quantifying the interaction between proteins and nucleic acids is of crucial interest in biological and chemical research. Here, we have made use of sitespecific fluorine labeling of the cold shock protein from Bacillus subtiliis , Bs CspB, enabling to directly monitor the interaction with single stranded DNA molecules in cell lysate. High-resolution 19 F NMR spectroscopy has been applied to exclusively report on resonance signals arising from the...
[NMR paper] Local deuteration enables NMR observation of methyl groups in proteins from eukaryotic and cell-free expression systems
Local deuteration enables NMR observation of methyl groups in proteins from eukaryotic and cell-free expression systems
Therapeutically relevant proteins such as GPCRs, antibodies and kinases face clear limitations in NMR studies due to the challenges in site-specific isotope labeling and deuteration in eukaryotic expression systems. Here we describe an efficient and simple method to observe the methyl groups of leucine residues in proteins expressed in bacterial, eukaryotic or cell-free expression systems without modification of the expression protocol. The method relies on simple...
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03-27-2021 02:09 AM
[NMR paper] Insights into protein stability in cell lysate by 19F NMR spectroscopy.
Insights into protein stability in cell lysate by 19F NMR spectroscopy.
Related Articles Insights into protein stability in cell lysate by 19F NMR spectroscopy.
Chembiochem. 2020 Aug 12;:
Authors: Welte H, Kovermann M
Abstract
In living organisms, protein folding and function take place in an inhomogeneous, highly crowded environment possessing a concentration of diverse macromolecules of up to 400*g/L. It has been shown that the intracellular environment has a pronounced effect on the stability, dynamics and function of the...
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08-15-2020 05:51 AM
[NMR paper] The precious fluorine on the ring: fluorine NMR for biological systems.
The precious fluorine on the ring: fluorine NMR for biological systems.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles The precious fluorine on the ring: fluorine NMR for biological systems.
J Biomol NMR. 2020 Jul 10;:
Authors: Boeszoermenyi A, Ogórek B, Jain A, Arthanari H, Wagner G
Abstract
The fluorine-19 nucleus was recognized early to harbor exceptional properties for NMR spectroscopy. With 100% natural abundance, a high...
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07-13-2020 01:56 AM
The Cation-? Interaction Enables a Halo-TagFluorogenic Probe for Fast No-Wash Live Cell Imaging and Gel-FreeProtein Quantification
The Cation-? Interaction Enables a Halo-TagFluorogenic Probe for Fast No-Wash Live Cell Imaging and Gel-FreeProtein Quantification
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.7b00056/20170308/images/medium/bi-2017-00056j_0007.gif
Biochemistry
DOI: 10.1021/acs.biochem.7b00056
http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/bichaw/~4/K_EHWBvChxU
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03-14-2017 08:16 AM
Affinity screening using competitive binding with fluorine-19 hyperpolarized ligands
From The DNP-NMR Blog:
Affinity screening using competitive binding with fluorine-19 hyperpolarized ligands
Kim, Y. and C. Hilty, Affinity screening using competitive binding with fluorine-19 hyperpolarized ligands. Angew Chem Int Ed Engl, 2015. 54(16): p. 4941-4.
http://www.ncbi.nlm.nih.gov/pubmed/25703090
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10-08-2015 12:56 AM
[NMR paper] Fluorine NMR-Based Screening on Cell Membrane Extracts.
Fluorine NMR-Based Screening on Cell Membrane Extracts.
Related Articles Fluorine NMR-Based Screening on Cell Membrane Extracts.
ChemMedChem. 2013 Dec 11;
Authors: Veronesi M, Romeo E, Lambruschini C, Piomelli D, Bandiera T, Scarpelli R, Garau G, Dalvit C
Abstract
The possibility of measuring the action of inhibitors of specific enzymatic reactions in intact cells, cell lysates or membrane preparations represents a major advance in the lead discovery process. Despite the relevance of assaying in physiological conditions, only a small...
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12-18-2013 04:00 PM
Matrix-free dynamic nuclear polarization enables solid-state NMR (13)C-(13)C correlation spectroscopy of proteins at natural isotopic abundance
From The DNP-NMR Blog:
Matrix-free dynamic nuclear polarization enables solid-state NMR (13)C-(13)C correlation spectroscopy of proteins at natural isotopic abundance
Takahashi, H., S. Hediger, and G. De Paepe, Matrix-free dynamic nuclear polarization enables solid-state NMR (13)C-(13)C correlation spectroscopy of proteins at natural isotopic abundance. Chem Commun (Camb), 2013. 49(82): p. 9479-81.
http://www.ncbi.nlm.nih.gov/pubmed/24013616
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11-21-2013 01:14 AM
[NMR paper] Matrix-free dynamic nuclear polarization enables solid-state NMR (13)C-(13)C correlation spectroscopy of proteins at natural isotopic abundance.
Matrix-free dynamic nuclear polarization enables solid-state NMR (13)C-(13)C correlation spectroscopy of proteins at natural isotopic abundance.
Matrix-free dynamic nuclear polarization enables solid-state NMR (13)C-(13)C correlation spectroscopy of proteins at natural isotopic abundance.
Chem Commun (Camb). 2013 Sep 6;
Authors: Takahashi H, Hediger S, De Paëpe G
Abstract
We introduce a general approach for dynamic nuclear polarization (DNP) enhanced solid-state NMR that overcomes the current problems in DNP experiments caused by the use...
Fluorine NMR spectroscopy enables to quantify the affinity between DNA and proteins in cell lysate
Linear Mode
