Fluorine-19 NMR of integral membrane proteins illustrated with studies of GPCRs
 

Fluorine-19 NMR of integral membrane proteins illustrated with studies of GPCRs

Publication date: Available online 7 August 2013
Source:Current Opinion in Structural Biology</br>
Author(s): Tatiana Didenko , Jeffrey J Liu , Reto Horst , Raymond C Stevens , Kurt Wüthrich</br>
Fluorine-19 is a spin-½ NMR isotope with high sensitivity and large chemical shift dispersion, which makes it attractive for high resolution NMR spectroscopy in solution. For studies of membrane proteins it is further of interest that 19F is rarely found in biological materials, which enables observation of extrinsic 19F labels with minimal interference from background signals. Today, after a period with rather limited use of 19F NMR in structural biology, we witness renewed interest in this technology for studies of complex supramolecular systems. Here we report on recent 19F NMR studies with the G protein-coupled receptor family of membrane proteins.
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