Hadamard encoded saturation transfer can significantly improve the efficiency of NOE-based NMR correlations from labile protons in proteins, glycans and RNAs, increasing the sensitivity of cross-peaks by an order of magnitude and shortening experimental times by >=100-fold. These schemes, however, fail when tackling correlations within a pool of labile protons -for instance imino-imino correlations in RNAs or amide-amide correlations in proteins. Here we analyze the origin of the artifacts...
[NMR paper] Heteronuclear transfers from labile protons in biomolecular NMR: Cross polarization, revisited
Heteronuclear transfers from labile protons in biomolecular NMR: Cross polarization, revisited
INEPT- and HMQC-based pulse sequences are widely used to transfer polarization between heteronuclei, particularly in biomolecular spectroscopy: they are easy to setup and involve low power deposition. Still, these short-pulse polarization transfers schemes are challenged by fast solvent chemical exchange. An alternative to improve these heteronuclear transfers is J-driven cross polarization (J-CP), which transfers polarization by spin-locking the coupled spins under Hartmann-Hahn conditions....
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nmrlearner
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10-25-2021 10:32 PM
[ASAP] The Incorporation of Labile Protons into Multidimensional NMR Analyses: Glycan Structures Revisited
The Incorporation of Labile Protons into Multidimensional NMR Analyses: Glycan Structures Revisited
Mihajlo Novakovic, Marcos D. Battistel, Hugo F. Azurmendi, Maria-Grazia Concilio, Daro?n I. Freedberg, and Lucio Frydman
https://pubs.acs.org/na101/home/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.1c04512/20210604/images/medium/ja1c04512_0008.gif
Journal of the American Chemical Society
DOI: 10.1021/jacs.1c04512
http://feeds.feedburner.com/~r/acs/jacsat/~4/H2MJ7chRt4g
nmrlearner
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06-05-2021 09:30 AM
[NMR paper] The Incorporation of Labile Protons into Multidimensional NMR Analyses: Glycan Structures Revisited
The Incorporation of Labile Protons into Multidimensional NMR Analyses: Glycan Structures Revisited
Glycan structures are often stabilized by a repertoire of hydrogen-bonded donor/acceptor groups, revealing longer-lived structures that could represent biologically relevant conformations. NMR provides unique data on these hydrogen-bonded networks from multidimensional experiments detecting cross-peaks resulting from through-bond (TOCSY) or through-space (NOESY) interactions. However, fast OH/H(2)O exchange, and the spectral proximity among these NMR resonances, hamper the use of glycans'...
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06-05-2021 09:30 AM
[NMR paper] Sensitivity enhancement of homonuclear multidimensional NMR correlations for labile sites in proteins, polysaccharides, and nucleic acids.
Sensitivity enhancement of homonuclear multidimensional NMR correlations for labile sites in proteins, polysaccharides, and nucleic acids.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.nature.com-images-lo_npg.gif http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:--www.nature.com-images-npg_logo.gif Sensitivity enhancement of homonuclear multidimensional NMR correlations for labile sites in proteins, polysaccharides, and nucleic acids.
Nat Commun. 2020 10 21;11(1):5317
Authors: Novakovic M,...
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10-24-2020 10:43 PM
Sensitivity-enhanced detection of non-labile proton and carbon NMR spectra on water resonances #DNPNMR
From The DNP-NMR Blog:
Sensitivity-enhanced detection of non-labile proton and carbon NMR spectra on water resonances #DNPNMR
Novakovic, M., et al., Sensitivity-enhanced detection of non-labile proton and carbon NMR spectra on water resonances. Phys. Chem. Chem. Phys., 2017. 20(1): p. 56-62.
https://www.ncbi.nlm.nih.gov/pubmed/29171604
nmrlearner
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02-19-2018 08:46 PM
[Question from NMRWiki Q&A forum] OH, NH, SH Labile protons in water nmr
OH, NH, SH Labile protons in water nmr
Hello all,
Just to mirror Craig Grimmer's question about labile protons in non-aqueous solvents, does anyone know of any good articles dealing with labile proton behavior in water?
Thanks,
nmrlearner
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03-11-2015 09:59 PM
Optimization of an absolute sensitivity in a glassy matrix during DNP-enhanced multidimensional solid-state NMR experiments
From The DNP-NMR Blog:
Optimization of an absolute sensitivity in a glassy matrix during DNP-enhanced multidimensional solid-state NMR experiments
Takahashi, H., et al., Optimization of an absolute sensitivity in a glassy matrix during DNP-enhanced multidimensional solid-state NMR experiments. J Magn Reson, 2013. 239C(0): p. 91-99.
http://www.ncbi.nlm.nih.gov/pubmed/24480716
nmrlearner
News from NMR blogs
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02-10-2014 08:46 PM
Labile proton assignment in DNA
can anybody help in assignment of exchangeable proton in DNA? I got stuck in assigning the labile proton in my DNA sequence. can anyone provide me the strategy of Labile proton assignment in DNA.
I would be highly thankful for.
thanks
The Extended Hadamard Transform: Sensitivity-Enhanced NMR Experiments Among Labile and Non-Labile 1Hs of SARS-CoV-2-derived RNAs
Linear Mode
