Examining the Transient Dark State in Protein-Quantum Dot Interaction by Relaxation-Based Solution NMR
Examining the Transient Dark State in Protein-Quantum Dot Interaction by Relaxation-Based Solution NMR
We probed the "dark" state involved in the protein-quantum dot (QD) interaction using a relaxation-based solution nuclear magnetic resonance (NMR) approach. We examined the dynamics and exchange kinetics of the ubiquitin-CdTe model system, which undergoes a fast exchange in the transverse relaxation time scale. We applied the recently developed dark-state exchange saturation transfer (DEST), lifetime line broadening (?R(2)), and exchange-induced chemical shift (?(ex)) solution NMR techniques to... More... |
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